A9KC41 (LPXB_COXBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	CBUD_0632

Organism

Coxiella burnetii (strain Dugway 5J108-111) [Complete proteome] [HAMAP]

Taxonomic identifier

434922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP MF_00392
Sequence similarities	Belongs to the LpxB family.
Sequence caution	The sequence ABS78090.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Lipid-A-disaccharide synthase HAMAP MF_00392		PRO_1000080276

Sequences

	Sequence		Length	Mass (Da)
	A9KC41 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 6D11A61B53A58E6E	FASTA	376	41,928
	10 20 30 40 50 60 MSNKSVLLIA GEPSGDLLGA HLAQSLKSLE PNLKLAGMGG KRMREAGVEV FINADKLAVV 70 80 90 100 110 120 GLLEILRQFR DIRHAMQTLK RYFKKTPPDL VVFIDYPGFN LHMAKQAKKA GIKVLYYVSP 130 140 150 160 170 180 QIWAWRYGRI KKIKKYVDHM AVLFDFEEKL YQKENVPVSF VGHPLANAPT PSLSRNEICK 190 200 210 220 230 240 QFNLDPDKPI VALFPGSREQ EINKLLPMMV QAGKLIQTQI PTVQFILPLA LNLALDKIRP 250 260 270 280 290 300 FLSPEIKVIQ NDISHVLAIA HAAVAASGTV TLEIALQQVP LVIIYKVAPL TFWLGKKLIR 310 320 330 340 350 360 LSFIGLCNLV SPEPVAVELL QQDATPQAIA DEVFQLLNNH NYRQSIIGKL GHLRPQLDRG 370 NAAQNVAKVV HNLIFS

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Dugway 5J108-111.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000733 Genomic DNA. Translation: ABS78090.2. Different initiation.
RefSeq	YP_001424039.2. NC_009727.1.
3D structure databases
ProteinModelPortal	A9KC41.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KC41.
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5459065.
GenomeReviews	Gene locus CBUD_0632 in contig CP000733_GR.
KEGG	cbd:CBUD_0632.
PATRIC	17921088. VBICoxBur32972_0660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG584837.
OMA	AEFAFIC.
ProtClustDB	PRK00025.
Family and domain databases
HAMAP	MF_00392. LpxB. [Tree]
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
KO	K00748.
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. LpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_COXBN

Accession

Primary (citable) accession number: A9KC41

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program