Reviewed, UniProtKB/Swiss-Prot A9KBJ3 (SYE1_COXBN)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	CBUD_0531

Organism

Coxiella burnetii (strain Dugway 5J108-111) [Complete proteome] [HAMAP]

Taxonomic identifier

434922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367659
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022
	Motif	249 – 253	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	252	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KBJ3-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 393FE9F270B9F55B	FASTA	465	52,691
	10 20 30 40 50 60 MMKSRFCPSP TGLMHLGNAR TALFNYLFAK SKDGIFLLRI EDTDVERSKE TFDLGLKEDL 70 80 90 100 110 120 RWLNLEWQEG PGADEGNGPY HQSKRQAIYD DYYQRLEEAD QAYPCFCSEE QLRLSRKIQR 130 140 150 160 170 180 SAGKPPRYAG TCRSLSAAEI EKKKAEGLQP ALRFRVPDDE VVVFADLVRG EQRFQTNDIG 190 200 210 220 230 240 DFIIRRANGT SPFMFCNAID DALMGVSHVL RGEDHLTNTP RQLLILQALE LPVPTYAHIA 250 260 270 280 290 300 LIVGPDGSPL SKRHGSRGIK ELRDNGYLPL ALTNYLARLG HYYASDELLS LAELAKGFNV 310 320 330 340 350 360 ESLSKSPAKF NAQQLDYWQK QTVNQLPNDD FWEWAGSELQ SQIPTDKADL FLTTVKPNAS 370 380 390 400 410 420 FPRDVAYWVN VCFGKTLNLE TAQSELLRAT GNRYFEEAFE AFKKFGKDLN SVVSHLKEKL 430 440 450 460 NLKGKPLYQP LRIALTGAEH GPELAKLILI MDYETIQNRL QEACQ

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000733 Genomic DNA. Translation: ABS78423.1.
RefSeq	YP_001423945.1.
3D structure databases
SMR	A9KBJ3. Positions 1-464.
ModBase	Search...
Genome annotation databases
GeneID	5457444.
GenomeReviews	Gene locus CBUD_0531 in contig CP000733_GR.
KEGG	cbd:CBUD_0531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	CNAIDDA.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_COXBN

Accession

Primary (citable) accession number: A9KBJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)