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A9JTS5

- STPAP_XENTR

UniProt

A9JTS5 - STPAP_XENTR

Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

tut1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.By similarity

    Catalytic activityi

    UTP + RNA(n) = diphosphate + RNA(n+1).
    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi226 – 2261Magnesium or manganese; catalyticBy similarity
    Metal bindingi228 – 2281Magnesium or manganese; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri14 – 3825C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. mRNA 3'-UTR binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB
    6. RNA uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. snRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
    Short name:
    Star-PAP
    Alternative name(s):
    RNA-binding motif protein 21
    Short name:
    RNA-binding protein 21
    U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
    Short name:
    U6-TUTase
    Gene namesi
    Name:tut1
    Synonyms:rbm21
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-491663. tut1.

    Subcellular locationi

    Nucleusnucleolus By similarity. Nucleus speckle By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 843843Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000404591Add
    BLAST

    Proteomic databases

    PaxDbiA9JTS5.

    Interactioni

    Subunit structurei

    Associates with the cleavage and polyadenylation specificity factor (CPSF) complex.By similarity

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000012439.

    Structurei

    3D structure databases

    ProteinModelPortaliA9JTS5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 12673RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini456 – 51661PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 PAP-associated domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri14 – 3825C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5260.
    HOGENOMiHOG000115998.
    HOVERGENiHBG079670.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9JTS5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEAEESSSSV VPLPQDVQSV VRGGFRCLLC GVNIPNRPSL TDHLSGRRHV    50
    RLHEERDKRN QQQERSVYVS NFPRETSEEQ LRDVFQKISP VRNIVMDKDR 100
    GLYAIVEFES KDGMCAALEE PQIKLSGKRL RVKPREKKEF QRKKGGSPRT 150
    LQPPDPEALS KELLNCADVE QQIKKLVSLC SPSHHESHLR ELLLSLLQET 200
    FTEFFPGCQL LPFGSSVNGF EISGCDLDLY LDLGDDEAEN VEGKAEKEIQ 250
    NREESSTDME VSMEDPETER KEEEMEIGNS KNDEDEDVTP GLSLKGLSSE 300
    EILEVVGKVL RHCVPGVHGV QSVPTARRPV IHFQHKTSGL RGDVTLNNRL 350
    ALRNSSFLRL CSDLDARVPQ LVYTVRYWAR VNQLAGNPFG GGPLLNNYAL 400
    TLLVFFFLQT RNPPVLPTLV HLREETANEV PQVIDGWDCS FPSDPAQVKE 450
    SGNQQSLSSL LSEFFSFYAS LDLHLLILCP CNGLTIPLPF SSPPPAWSEG 500
    FRLGPLNIQD PFELSHNVCG NVSSRAARRF ISHCAAAARI CRTPNYNLHS 550
    TSHPWGITPI LLPPPTEREC VGRGGTEISI PLGGVSPEKT YAAVSKVFVD 600
    VLLCTLEEGR EDSCQEGKAL ELSTKHAKAQ CKVEKNEVGG ELGEQEVPCK 650
    AEQNNTKEAS KQKSIFKTEE GMTESARRKR EMTEPCMSDM TNGKKRRLEF 700
    TRGIWDHHLA TSAMEEEMCG EAHKDSKTKI DYSNNGTAQW ELLVWHRVWE 750
    GRRKERRRKQ KGEADGVELE IAVSQALALE KEDKCDGPLM KLILTAQLTV 800
    KESLQLYLTP KFDPQGLSST FFHFLESYLP RMVAQIQGCG DPV 843
    Length:843
    Mass (Da):94,496
    Last modified:February 5, 2008 - v1
    Checksum:iB36785E0C7A5A4CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC155457 mRNA. Translation: AAI55458.1.
    RefSeqiXP_002941502.2. XM_002941456.2.
    UniGeneiStr.66101.

    Genome annotation databases

    GeneIDi100127188.
    KEGGixtr:100127188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC155457 mRNA. Translation: AAI55458.1 .
    RefSeqi XP_002941502.2. XM_002941456.2.
    UniGenei Str.66101.

    3D structure databases

    ProteinModelPortali A9JTS5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000012439.

    Proteomic databases

    PaxDbi A9JTS5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100127188.
    KEGGi xtr:100127188.

    Organism-specific databases

    CTDi 64852.
    Xenbasei XB-GENE-491663. tut1.

    Phylogenomic databases

    eggNOGi COG5260.
    HOGENOMi HOG000115998.
    HOVERGENi HBG079670.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: TGA IC.
      Tissue: Testis.

    Entry informationi

    Entry nameiSTPAP_XENTR
    AccessioniPrimary (citable) accession number: A9JTS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3