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A9JTS5 (STPAP_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase

Short name=Star-PAP
EC=2.7.7.19
Alternative name(s):
RNA-binding motif protein 21
Short name=RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1
Short name=U6-TUTase
EC=2.7.7.52
Gene names
Name:tut1
Synonyms:rbm21
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length843 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.

Catalytic activity

UTP + RNA(n) = diphosphate + RNA(n+1).

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex By similarity.

Subcellular location

Nucleusnucleolus By similarity. Nucleus speckle By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B-like family.

Contains 1 C2H2-type zinc finger.

Contains 1 PAP-associated domain.

Contains 1 RRM (RNA recognition motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 843843Speckle targeted PIP5K1A-regulated poly(A) polymerase
PRO_0000404591

Regions

Domain54 – 12673RRM
Domain456 – 51661PAP-associated
Zinc finger14 – 3825C2H2-type

Sites

Metal binding2261Magnesium or manganese; catalytic By similarity
Metal binding2281Magnesium or manganese; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
A9JTS5 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: B36785E0C7A5A4CC

FASTA84394,496
        10         20         30         40         50         60 
MEAEESSSSV VPLPQDVQSV VRGGFRCLLC GVNIPNRPSL TDHLSGRRHV RLHEERDKRN 

        70         80         90        100        110        120 
QQQERSVYVS NFPRETSEEQ LRDVFQKISP VRNIVMDKDR GLYAIVEFES KDGMCAALEE 

       130        140        150        160        170        180 
PQIKLSGKRL RVKPREKKEF QRKKGGSPRT LQPPDPEALS KELLNCADVE QQIKKLVSLC 

       190        200        210        220        230        240 
SPSHHESHLR ELLLSLLQET FTEFFPGCQL LPFGSSVNGF EISGCDLDLY LDLGDDEAEN 

       250        260        270        280        290        300 
VEGKAEKEIQ NREESSTDME VSMEDPETER KEEEMEIGNS KNDEDEDVTP GLSLKGLSSE 

       310        320        330        340        350        360 
EILEVVGKVL RHCVPGVHGV QSVPTARRPV IHFQHKTSGL RGDVTLNNRL ALRNSSFLRL 

       370        380        390        400        410        420 
CSDLDARVPQ LVYTVRYWAR VNQLAGNPFG GGPLLNNYAL TLLVFFFLQT RNPPVLPTLV 

       430        440        450        460        470        480 
HLREETANEV PQVIDGWDCS FPSDPAQVKE SGNQQSLSSL LSEFFSFYAS LDLHLLILCP 

       490        500        510        520        530        540 
CNGLTIPLPF SSPPPAWSEG FRLGPLNIQD PFELSHNVCG NVSSRAARRF ISHCAAAARI 

       550        560        570        580        590        600 
CRTPNYNLHS TSHPWGITPI LLPPPTEREC VGRGGTEISI PLGGVSPEKT YAAVSKVFVD 

       610        620        630        640        650        660 
VLLCTLEEGR EDSCQEGKAL ELSTKHAKAQ CKVEKNEVGG ELGEQEVPCK AEQNNTKEAS 

       670        680        690        700        710        720 
KQKSIFKTEE GMTESARRKR EMTEPCMSDM TNGKKRRLEF TRGIWDHHLA TSAMEEEMCG 

       730        740        750        760        770        780 
EAHKDSKTKI DYSNNGTAQW ELLVWHRVWE GRRKERRRKQ KGEADGVELE IAVSQALALE 

       790        800        810        820        830        840 
KEDKCDGPLM KLILTAQLTV KESLQLYLTP KFDPQGLSST FFHFLESYLP RMVAQIQGCG 


DPV 

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References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: TGA IC.
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC155457 mRNA. Translation: AAI55458.1.
RefSeqXP_002941502.2. XM_002941456.2.
UniGeneStr.66101.

3D structure databases

ProteinModelPortalA9JTS5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000012439.

Proteomic databases

PaxDbA9JTS5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100127188.
KEGGxtr:100127188.

Organism-specific databases

CTD64852.
XenbaseXB-GENE-491663. tut1.

Phylogenomic databases

eggNOGCOG5260.
HOGENOMHOG000115998.
HOVERGENHBG079670.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSTPAP_XENTR
AccessionPrimary (citable) accession number: A9JTS5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families