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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

tut1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase (By similarity).By similarity

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi226 – 2261Magnesium or manganese; catalyticBy similarity
Metal bindingi228 – 2281Magnesium or manganese; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:tut1
Synonyms:rbm21
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-491663. tut1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 843843Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000404591Add
BLAST

Proteomic databases

PaxDbiA9JTS5.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex.By similarity

Protein-protein interaction databases

STRINGi8364.ENSXETP00000012439.

Structurei

3D structure databases

ProteinModelPortaliA9JTS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 12673RRMPROSITE-ProRule annotationAdd
BLAST
Domaini456 – 51661PAP-associatedAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 1 C2H2-type zinc finger.Curated
Contains 1 PAP-associated domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
HOGENOMiHOG000115998.
HOVERGENiHBG079670.
InParanoidiA9JTS5.
KOiK18709.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9JTS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAEESSSSV VPLPQDVQSV VRGGFRCLLC GVNIPNRPSL TDHLSGRRHV
60 70 80 90 100
RLHEERDKRN QQQERSVYVS NFPRETSEEQ LRDVFQKISP VRNIVMDKDR
110 120 130 140 150
GLYAIVEFES KDGMCAALEE PQIKLSGKRL RVKPREKKEF QRKKGGSPRT
160 170 180 190 200
LQPPDPEALS KELLNCADVE QQIKKLVSLC SPSHHESHLR ELLLSLLQET
210 220 230 240 250
FTEFFPGCQL LPFGSSVNGF EISGCDLDLY LDLGDDEAEN VEGKAEKEIQ
260 270 280 290 300
NREESSTDME VSMEDPETER KEEEMEIGNS KNDEDEDVTP GLSLKGLSSE
310 320 330 340 350
EILEVVGKVL RHCVPGVHGV QSVPTARRPV IHFQHKTSGL RGDVTLNNRL
360 370 380 390 400
ALRNSSFLRL CSDLDARVPQ LVYTVRYWAR VNQLAGNPFG GGPLLNNYAL
410 420 430 440 450
TLLVFFFLQT RNPPVLPTLV HLREETANEV PQVIDGWDCS FPSDPAQVKE
460 470 480 490 500
SGNQQSLSSL LSEFFSFYAS LDLHLLILCP CNGLTIPLPF SSPPPAWSEG
510 520 530 540 550
FRLGPLNIQD PFELSHNVCG NVSSRAARRF ISHCAAAARI CRTPNYNLHS
560 570 580 590 600
TSHPWGITPI LLPPPTEREC VGRGGTEISI PLGGVSPEKT YAAVSKVFVD
610 620 630 640 650
VLLCTLEEGR EDSCQEGKAL ELSTKHAKAQ CKVEKNEVGG ELGEQEVPCK
660 670 680 690 700
AEQNNTKEAS KQKSIFKTEE GMTESARRKR EMTEPCMSDM TNGKKRRLEF
710 720 730 740 750
TRGIWDHHLA TSAMEEEMCG EAHKDSKTKI DYSNNGTAQW ELLVWHRVWE
760 770 780 790 800
GRRKERRRKQ KGEADGVELE IAVSQALALE KEDKCDGPLM KLILTAQLTV
810 820 830 840
KESLQLYLTP KFDPQGLSST FFHFLESYLP RMVAQIQGCG DPV
Length:843
Mass (Da):94,496
Last modified:February 5, 2008 - v1
Checksum:iB36785E0C7A5A4CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC155457 mRNA. Translation: AAI55458.1.
RefSeqiXP_002941502.2. XM_002941456.2.
UniGeneiStr.66101.

Genome annotation databases

GeneIDi100127188.
KEGGixtr:100127188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC155457 mRNA. Translation: AAI55458.1.
RefSeqiXP_002941502.2. XM_002941456.2.
UniGeneiStr.66101.

3D structure databases

ProteinModelPortaliA9JTS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000012439.

Proteomic databases

PaxDbiA9JTS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100127188.
KEGGixtr:100127188.

Organism-specific databases

CTDi64852.
XenbaseiXB-GENE-491663. tut1.

Phylogenomic databases

eggNOGiCOG5260.
HOGENOMiHOG000115998.
HOVERGENiHBG079670.
InParanoidiA9JTS5.
KOiK18709.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: TGA IC.
    Tissue: Testis.

Entry informationi

Entry nameiSTPAP_XENTR
AccessioniPrimary (citable) accession number: A9JTS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: February 5, 2008
Last modified: May 27, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.