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A9JTS5

- STPAP_XENTR

UniProt

A9JTS5 - STPAP_XENTR

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Protein
Speckle targeted PIP5K1A-regulated poly(A) polymerase
Gene
tut1, rbm21
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi226 – 2261Magnesium or manganese; catalytic By similarity
Metal bindingi228 – 2281Magnesium or manganese; catalytic By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-type
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB
  3. RNA uridylyltransferase activity Source: UniProtKB
  4. mRNA 3'-UTR binding Source: UniProtKB
  5. metal ion binding Source: UniProtKB-KW
  6. polynucleotide adenylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:tut1
Synonyms:rbm21
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-491663. tut1.

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 843843Speckle targeted PIP5K1A-regulated poly(A) polymerase
PRO_0000404591Add
BLAST

Proteomic databases

PaxDbiA9JTS5.

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex By similarity.

Protein-protein interaction databases

STRINGi8364.ENSXETP00000012439.

Structurei

3D structure databases

ProteinModelPortaliA9JTS5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 12673RRM
Add
BLAST
Domaini456 – 51661PAP-associated
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
HOGENOMiHOG000115998.
HOVERGENiHBG079670.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9JTS5-1 [UniParc]FASTAAdd to Basket

« Hide

MEAEESSSSV VPLPQDVQSV VRGGFRCLLC GVNIPNRPSL TDHLSGRRHV    50
RLHEERDKRN QQQERSVYVS NFPRETSEEQ LRDVFQKISP VRNIVMDKDR 100
GLYAIVEFES KDGMCAALEE PQIKLSGKRL RVKPREKKEF QRKKGGSPRT 150
LQPPDPEALS KELLNCADVE QQIKKLVSLC SPSHHESHLR ELLLSLLQET 200
FTEFFPGCQL LPFGSSVNGF EISGCDLDLY LDLGDDEAEN VEGKAEKEIQ 250
NREESSTDME VSMEDPETER KEEEMEIGNS KNDEDEDVTP GLSLKGLSSE 300
EILEVVGKVL RHCVPGVHGV QSVPTARRPV IHFQHKTSGL RGDVTLNNRL 350
ALRNSSFLRL CSDLDARVPQ LVYTVRYWAR VNQLAGNPFG GGPLLNNYAL 400
TLLVFFFLQT RNPPVLPTLV HLREETANEV PQVIDGWDCS FPSDPAQVKE 450
SGNQQSLSSL LSEFFSFYAS LDLHLLILCP CNGLTIPLPF SSPPPAWSEG 500
FRLGPLNIQD PFELSHNVCG NVSSRAARRF ISHCAAAARI CRTPNYNLHS 550
TSHPWGITPI LLPPPTEREC VGRGGTEISI PLGGVSPEKT YAAVSKVFVD 600
VLLCTLEEGR EDSCQEGKAL ELSTKHAKAQ CKVEKNEVGG ELGEQEVPCK 650
AEQNNTKEAS KQKSIFKTEE GMTESARRKR EMTEPCMSDM TNGKKRRLEF 700
TRGIWDHHLA TSAMEEEMCG EAHKDSKTKI DYSNNGTAQW ELLVWHRVWE 750
GRRKERRRKQ KGEADGVELE IAVSQALALE KEDKCDGPLM KLILTAQLTV 800
KESLQLYLTP KFDPQGLSST FFHFLESYLP RMVAQIQGCG DPV 843
Length:843
Mass (Da):94,496
Last modified:February 5, 2008 - v1
Checksum:iB36785E0C7A5A4CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC155457 mRNA. Translation: AAI55458.1.
RefSeqiXP_002941502.2. XM_002941456.2.
UniGeneiStr.66101.

Genome annotation databases

GeneIDi100127188.
KEGGixtr:100127188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC155457 mRNA. Translation: AAI55458.1 .
RefSeqi XP_002941502.2. XM_002941456.2.
UniGenei Str.66101.

3D structure databases

ProteinModelPortali A9JTS5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000012439.

Proteomic databases

PaxDbi A9JTS5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100127188.
KEGGi xtr:100127188.

Organism-specific databases

CTDi 64852.
Xenbasei XB-GENE-491663. tut1.

Phylogenomic databases

eggNOGi COG5260.
HOGENOMi HOG000115998.
HOVERGENi HBG079670.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: TGA IC.
    Tissue: Testis.

Entry informationi

Entry nameiSTPAP_XENTR
AccessioniPrimary (citable) accession number: A9JTS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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