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A9JSE7

- MIF_XENTR

UniProt

A9JSE7 - MIF_XENTR

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Protein
Macrophage migration inhibitory factor
Gene
mif
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogen By similarity
Binding sitei33 – 331Substrate By similarity
Binding sitei65 – 651Substrate; via amide nitrogen By similarity
Binding sitei98 – 981Substrate By similarity

GO - Molecular functioni

  1. dopachrome isomerase activity Source: UniProtKB-EC
  2. phenylpyruvate tautomerase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. inflammatory response Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:mif
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-487448. mif.

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 115114Macrophage migration inhibitory factor
PRO_0000344371Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliA9JSE7.
SMRiA9JSE7. Positions 1-115.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.

Phylogenomic databases

HOVERGENiHBG003240.
KOiK07253.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9JSE7-1 [UniParc]FASTAAdd to Basket

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MPTFTVCTNV CRDSMPDTLL SDLTKLLAKA TGKPAEYIAI HIMPDQMMSF    50
GDSTDPCAVC SLSSIGKIGG PQNKSYSKLL CDYLTKQMNI PANRVYINFH 100
DLNPANVGWN GSTFA 115
Length:115
Mass (Da):12,524
Last modified:February 5, 2008 - v1
Checksum:iF8EEDB313BF97710
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC156031 mRNA. Translation: AAI56032.1.
RefSeqiNP_001107147.1. NM_001113675.1.
UniGeneiStr.22164.

Genome annotation databases

GeneIDi100134996.
KEGGixtr:100134996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC156031 mRNA. Translation: AAI56032.1 .
RefSeqi NP_001107147.1. NM_001113675.1.
UniGenei Str.22164.

3D structure databases

ProteinModelPortali A9JSE7.
SMRi A9JSE7. Positions 1-115.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100134996.
KEGGi xtr:100134996.

Organism-specific databases

CTDi 4282.
Xenbasei XB-GENE-487448. mif.

Phylogenomic databases

HOVERGENi HBG003240.
KOi K07253.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiMIF_XENTR
AccessioniPrimary (citable) accession number: A9JSE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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