Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylthioribose-1-phosphate isomerase

Gene

mri1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).UniRule annotation

Catalytic activityi

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mri1)
  2. Methylthioribulose-1-phosphate dehydratase (apip), Methylthioribulose-1-phosphate dehydratase (apip), Methylthioribulose-1-phosphate dehydratase (apip), Methylthioribulose-1-phosphate dehydratase (apip), Methylthioribulose-1-phosphate dehydratase (apip), Methylthioribulose-1-phosphate dehydratase (apip)
  3. Enolase-phosphatase E1 (enoph1), Enolase-phosphatase E1 (enoph1)
  4. Enolase-phosphatase E1 (enoph1), Enolase-phosphatase E1 (enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1611Transition state stabilizerUniRule annotation
Active sitei241 – 2411Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

ReactomeiR-DRE-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00874.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose-1-phosphate isomeraseUniRule annotation (EC:5.3.1.23UniRule annotation)
Short name:
M1PiUniRule annotation
Short name:
MTR-1-P isomeraseUniRule annotation
Alternative name(s):
S-methyl-5-thioribose-1-phosphate isomeraseUniRule annotation
Translation initiation factor eIF-2B subunit alpha/beta/delta-like proteinUniRule annotation
Gene namesi
Name:mri1
ORF Names:zgc:172216
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-080204-109. mri1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Methylthioribose-1-phosphate isomerasePRO_0000401971Add
BLAST

Proteomic databases

PaxDbiA9JRE2.
PeptideAtlasiA9JRE2.

Expressioni

Gene expression databases

BgeeiA9JRE2.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000098757.

Structurei

3D structure databases

ProteinModelPortaliA9JRE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG1468. Eukaryota.
COG0182. LUCA.
GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
HOVERGENiHBG100263.
InParanoidiA9JRE2.
KOiK08963.
OMAiKVFNPAF.
PhylomeDBiA9JRE2.
TreeFamiTF300852.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.

Sequencei

Sequence statusi: Complete.

A9JRE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEAIRYRS GSLQILNQLL LPRETVYDEI RSVRDGYEAI KSMKVRGAPA
60 70 80 90 100
IAIVGCLSLA VELRAGAGAE DLVSFVRDSL CHLTSARPTA VNMGRAAREL
110 120 130 140 150
MEFTENESME KNTEQLRDSV IGWIEEMLER DVNDNKKIGN YGAQHILSGV
160 170 180 190 200
PRDSVTILTH CNTGSLATAG YGTALGVVRS LHMLGRLKRL YCTETRPYNQ
210 220 230 240 250
GARLTAYEAV AEGFPATLIT DSMAALAMRE KSITAVVVGA DRVVANGDTA
260 270 280 290 300
NKVGTYQLAI AAKHHGIPFY VAAPSTSCDL SLESGRDIVI EERPAEELTS
310 320 330 340 350
INGVPVAAPG IDVWNPAFDV TPHQLITGGI ITELGVFLPS ELQAALTGRL

TAL
Length:353
Mass (Da):37,980
Last modified:February 5, 2008 - v1
Checksum:iDE2CF09DC849F475
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC155626 mRNA. Translation: AAI55627.1.
BC171374 mRNA. Translation: AAI71374.1.
BC171376 mRNA. Translation: AAI71376.1.
RefSeqiNP_001104708.1. NM_001111238.1.
UniGeneiDr.85012.

Genome annotation databases

EnsembliENSDART00000111671; ENSDARP00000098757; ENSDARG00000075754.
GeneIDi100002302.
KEGGidre:100002302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC155626 mRNA. Translation: AAI55627.1.
BC171374 mRNA. Translation: AAI71374.1.
BC171376 mRNA. Translation: AAI71376.1.
RefSeqiNP_001104708.1. NM_001111238.1.
UniGeneiDr.85012.

3D structure databases

ProteinModelPortaliA9JRE2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000098757.

Proteomic databases

PaxDbiA9JRE2.
PeptideAtlasiA9JRE2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000111671; ENSDARP00000098757; ENSDARG00000075754.
GeneIDi100002302.
KEGGidre:100002302.

Organism-specific databases

CTDi84245.
ZFINiZDB-GENE-080204-109. mri1.

Phylogenomic databases

eggNOGiKOG1468. Eukaryota.
COG0182. LUCA.
GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
HOVERGENiHBG100263.
InParanoidiA9JRE2.
KOiK08963.
OMAiKVFNPAF.
PhylomeDBiA9JRE2.
TreeFamiTF300852.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00874.
ReactomeiR-DRE-1237112. Methionine salvage pathway.

Miscellaneous databases

PROiA9JRE2.

Gene expression databases

BgeeiA9JRE2.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiMTNA_DANRE
AccessioniPrimary (citable) accession number: A9JRE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: February 5, 2008
Last modified: July 6, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.