A9JPF0 (AGLB_HALVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase AglB
EC=2.4.1.119

Alternative name(s):
Archaeal glycosylation protein B
S-layer biosynthesis oligosaccharyl transferase AglB

Gene names

Name:

aglB

Organism

Haloferax volcanii (Halobacterium volcanii)

Taxonomic identifier

2246 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax

Protein attributes

Sequence length	1054 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in N-glycosylation of the S-layer glycoprotein. Contributes to the assembly of the pentasaccharide decorating at least 2 select Asn residues of the S-layer glycoprotein. Probably transfers the pentasaccharide from a dolichol phosphate carrier to at least 2 S-layer glycoprotein Asn residues, 'Asn-47' and 'Asn-117'.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Cell surface structure biogenesis; S-layer biogenesis. Protein modification; protein glycosylation.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the STT3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1054	1054	Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase AglB		PRO_0000415385
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	175 – 195	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	264 – 284	21	Helical; Potential
	Transmembrane	296 – 316	21	Helical; Potential
	Transmembrane	332 – 352	21	Helical; Potential
	Transmembrane	397 – 417	21	Helical; Potential
	Transmembrane	432 – 452	21	Helical; Potential
	Transmembrane	456 – 476	21	Helical; Potential
	Transmembrane	486 – 506	21	Helical; Potential
	Transmembrane	507 – 527	21	Helical; Potential
	Transmembrane	545 – 565	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A9JPF0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 855F63B0810835E8	FASTA	1,054	113,675
	10 20 30 40 50 60 MSDEQTKYSP SIAELARDWY HIPVLSTIIL VMLWIRLRSY DAFIREGTVF FSGNDAWYHL 70 80 90 100 110 120 RQVEYTVRNW PATMPFDPWT EFPFGRTAGQ FGTIYDQLVA TAALVVGLGS PSSDLVAKSL 130 140 150 160 170 180 LVAPAVFGAL TVIPTYLIGK RLGGRLGGLF GAVILMLLPG TFLQRGLVGF ADHNIVEPFF 190 200 210 220 230 240 MGFAVLAIMI ALTVADREKP VWELVAARDL DALREPLKWS VLAGVATAIY MWSWPPGILL 250 260 270 280 290 300 VGIFGLFLVL KMASDYVRGR SPEHTAFVGA ISMTVTGLLM FIPIEEPGFG VTDFGFLQPL 310 320 330 340 350 360 FSLGVALGAV FLAALARWWE SNDVDERYYP AVVGGTMLVG IVLFSLVLPS VFDSIARNFL 370 380 390 400 410 420 RTVGFSAGAA TRTISEAQPF LAANVLQSNG QTAVGRIMSE YGFTFFTGAL AAVWLVAKPL 430 440 450 460 470 480 VKGGNSRKIG YAVGSLALIG VLFLIPALPA GIGSALGVEP SLVSLTIVTA LIVGAVMQAD 490 500 510 520 530 540 YESERLFVLV WAAIITSAAF TQVRFNYYLA VVVAVMNAYL LREALGIDFV GLANVERFDD 550 560 570 580 590 600 ISYGQVAAVV IAVLLILTPV LIIPIQLGNG GVSQTAMQAS QTGPGTVTQW DGSLTWMQNN 610 620 630 640 650 660 TPAEGEFGGE SNRMEYYGTY EYTDDFDYPD GAYGVMSWWD YGHWITVLGE RIPNANPFQG 670 680 690 700 710 720 GATEAANYLL AEDEQQAESV LTSMGDDGEG DQTRYVMVDW QMASTDAKFS APTVFYDESN 730 740 750 760 770 780 ISRSDFYNPM FRLQEQGEQT TVAAASSLKD QRYYESLMVR LYAYHGSARE ASPIVVDWEE 790 800 810 820 830 840 RTSADGSTTF RVTPSDGQAV RTFDNMSAAE EYVANDPTSQ IGGIGTFPEE RVSALEHYRL 850 860 870 880 890 900 VKSSNSSALR SGSYQRSLIS EGNTYGLQPQ ALVPNNPAWV KTFERVPGAT VDGSGAPANT 910 920 930 940 950 960 TVTARVQMRD LTTGTNFTYT QQAQTDADGE FTMTLPYSTT GYDEYGPDNG YTNVSVRAAG 970 980 990 1000 1010 1020 GYAFTGPTSV TGNSTIVSYQ AENVAVDEGL VNGAEDGTVQ VTLERNEQEL DLPGDSSSED 1030 1040 1050 SSSEDGTSDG SQTNESASTS TSASVDASAV SAAA

References

[1]

"Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer." Abu-Qarn M., Yurist-Doutsch S., Giordana A., Trauner A., Morris H.R., Hitchen P., Medalia O., Dell A., Eichler J. J. Mol. Biol. 374:1224-1236(2007) [PubMed: 17996897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN GLYCOSYLATION, PATHWAY, GENE NAME. Strain: WR536 / H53 / DS70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM922226 Genomic DNA. Translation: CAP58184.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT66. Glycosyltransferase Family 66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

AGLB_HALVO

Accession

Primary (citable) accession number: A9JPF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program