A9IZV5 (MDH_BART1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	BT_2679

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Malate dehydrogenase HAMAP MF_00487		PRO_1000081353
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IZV5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 70C2D9A043AF03C6	FASTA	320	33,998
	10 20 30 40 50 60 MVRKKIALIG SGMIGGTLAH IIGLKELGDV VLFDISEGMP QGKALDIAES SPIDGFDVHL 70 80 90 100 110 120 KGANAYEAIE GADVVIVTAG VARKPGMSRD DLLGINLKVM EQVGAGIKKY APSAFVICIT 130 140 150 160 170 180 NPLDAMVWAL QKFSGLPVHK VVGMAGILDS ARFRYFLSEE FKVSVKDVTA FVLGGHGDSM 190 200 210 220 230 240 VPLVRYSTVG GISLPDLVKM GWTTQERIDQ IIQRTRDGGA EVISLLKTGS AYYAPAASAV 250 260 270 280 290 300 SMAEAYLKGT KRVVPVAAYL SGEYGVNDTY VGVPVVLGSG GVERVIEIDL DKEERDAFDY 310 320 SVNAVKKLCE ACIALVPSLK

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIP 105476 / IBS 506.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK02626.1.
RefSeq	YP_001610621.1. NC_010161.1.
3D structure databases
ProteinModelPortal	A9IZV5.
SMR	A9IZV5. Positions 3-312.
ModBase	Search...
Protein-protein interaction databases
STRING	A9IZV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5829603.
GenomeReviews	Gene locus BT_2679 in contig AM260525_GR.
PATRIC	20553222. VBIBarTri113218_2727.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	ANSYEAI.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	BTRI382640:BT_2679-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BART1

Accession

Primary (citable) accession number: A9IZV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program