Reviewed, UniProtKB/Swiss-Prot A9IWA5 (MURG_BART1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
    EC=2.4.1.227
Alternative name(s):
    Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase

Gene names

Name:	murG
Ordered Locus Names:	BT_1591

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity. HAMAP MF_00033
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00033
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00033
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_00033.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase HAMAP MF_00033		PRO_1000074447

Sequences

	Sequence		Length	Mass (Da)
	A9IWA5-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 2C2E7EADD69CE08E	FASTA	378	41,184
	10 20 30 40 50 60 MNDKKVIVLA AGGTGGHLFP AEALAVELRQ RGYDVHLITD ERARSFVRRF DEEHTHIVSS 70 80 90 100 110 120 ATFTRCHPFA LIKTILSLLK GMGQSLRLFY KLRPVLVGGF GGYPSFPPLF IAVLMRRVTF 130 140 150 160 170 180 IHEQNAVMGR ANRVLAIFVR AIAGGLLSPK GAYAHKTLLT GNPVREAVLK AAEIPYHSPV 190 200 210 220 230 240 GEEPFNFLVF GGSQGASFFS HIVPEAVALL DDQNRKRLRI VQQVRGDAAG LIKIYRDMGV 250 260 270 280 290 300 QAEVAPFFDD MAERMAHAHF ILSRAGASSV CEIAVIGRPA LLIPYPYALD YDQAENAALL 310 320 330 340 350 360 ARVGGAQIIS EKDLSAQKLA ALLTQACCAP HLLEKQALAA KKVGQPYATR CLADMAEALI 370 AGRLLSDIKE ELFDENAT

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01930.1.
RefSeq	YP_001609925.1.
3D structure databases
SMR	A9IWA5. Positions 6-359.
ModBase	Search...
Genome annotation databases
GeneID	5829024.
GenomeReviews	Gene locus BT_1591 in contig AM260525_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG617076.
OMA	GGTGGHM.
Family and domain databases
HAMAP	MF_00033. MurG. [Tree]
InterPro	IPR006009. GlcNAc_MurG. IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
TIGRFAMs	TIGR01133. murG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURG_BART1

Accession

Primary (citable) accession number: A9IWA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)