Reviewed, UniProtKB/Swiss-Prot A9IWA2 (DDL_BART1)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	BT1588

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	D-alanine--D-alanine ligase		PRO_1000074763
Regions
	Domain	102 – 300	199	ATP-grasp
	Nucleotide binding	128 – 183	56	ATP By similarity
Sites
	Metal binding	253	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 2 By similarity
	Metal binding	269	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IWA2-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: F4C9E32F42118FAC	FASTA	306	33,289
	10 20 30 40 50 60 MKDKHIAVLM GGFSSERSIS LSSGAACADV LEEQGYRVSR VDVGAHIASV LEQLQPDIAF 70 80 90 100 110 120 NALHGPFGED GRIQGILEYL KIPYTHSGVM ASALAMDKGR AKIIAANAGV CVAPSRIMNR 130 140 150 160 170 180 FSIGKTHPME PPYVIKPVCE GSSFGVVIVQ ENEAVPPHNI GGSEWGYADE VMVEKYIPGR 190 200 210 220 230 240 ELTCAVLGNE VLDVCEILPD QHFAFYDYDS KYKTGGSLHI CPAKLSLNIY QSVQRMSLAA 250 260 270 280 290 300 HQAIGCRGVS RSDFRFDEKT GELVWLEINT QPGMTSTSLL PDIAKASGRT YGDIVQWMVE DASCMR

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM260525 Genomic DNA. Translation: CAK01927.1.
RefSeq	YP_001609922.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5829274.
GenomeReviews	Gene locus BT1588 in contig AM260525_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR005905. D_ala_D_ala. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BART1

Accession

Primary (citable) accession number: A9IWA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)