Reviewed, UniProtKB/Swiss-Prot A9IW82 (TYSY_BART1)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BT_1568

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_1000073868
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IW82-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E55430172FD7D5DB	FASTA	264	30,378
	10 20 30 40 50 60 MKSYLALLSH VLNQGIDRTD RTGVGTRSIF GYQMRFDLQA GFPLLTTKKL HLRSIIYELL 70 80 90 100 110 120 WFLKGDTNIA WLKEHGVSIW DEWADKQGNL GPVYGYQWRS WPAPDGRHID QIDNLLRMIK 130 140 150 160 170 180 EKPDSRRLIV SAWNPALIEE MALPPCHCLF QFYIDEGKLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTMMIAQ VSGLKVGDFI HTFGDAHLYS NHFEQAQYQL SRIPNALPCM RINPAVTDLF 250 260 SFKFEDFELL NYEAHPHIKA PVAI

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01907.1.
RefSeq	YP_001609902.1.
3D structure databases
SMR	A9IW82. Positions 1-264.
ModBase	Search...
Genome annotation databases
GeneID	5829477.
GenomeReviews	Gene locus BT_1568 in contig AM260525_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	RMALAPC.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BART1

Accession

Primary (citable) accession number: A9IW82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)