A9IVI2 (SYD_BART1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BT_1358

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000074691

Sequences

	Sequence		Length	Mass (Da)
	A9IVI2 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 07CFBE3C94D63227	FASTA	597	67,738
	10 20 30 40 50 60 MHRYRSHHCA ALRKCDVGTK VRLSGWVHRV RDHGGILFVD LRDHFGITQI VADPASPAFK 70 80 90 100 110 120 VIEKVRSEWV IRVDGEVCAR SDEVINATLP TGEIEIFVQE VEILSKSEEL PLPVFGEPDY 130 140 150 160 170 180 PEDIRLKYRF LDLRRETMHK NIMRRTEIIA AMRRAMQNNG FTEFSTPLLT ASSPEGARDF 190 200 210 220 230 240 LVPSRVHQGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFVEQEDVL ATMEPIMRSI FEEFANGKTV TQNFPRISYD EAIQKYGSDK PDLRNPIIME 310 320 330 340 350 360 DVSQHFYNSG FKVFAQILAD DENAQVWAIP AKTGGSRAFC DRMNVWAQGE GQPGLGYIFW 370 380 390 400 410 420 RKEEEKFEGA GPIAKNIGEQ RTEALRIQLG LENGDACFFV AGDPKKFASF AGAARTRVGE 430 440 450 460 470 480 ELDLVDRECF SLAWIVDFPF FEWNEDEKKI DFAHNPFSMP QGGENALECQ DPLTLKAFQY 490 500 510 520 530 540 DLVCNGYEIA SGGIRNHLPE MMLKVFELVG LSKKVVKDRF GALYRAFHYG APPHGGMAAG 550 560 570 580 590 IDRIIMLLQG VKNLREVALF PMNQQALDLL MNAPSDVSST QLRDLGIRMA PTQKNSS

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIP 105476 / IBS 506.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01720.1.
RefSeq	YP_001609715.1. NC_010161.1.
3D structure databases
ProteinModelPortal	A9IVI2.
ModBase	Search...
Protein-protein interaction databases
STRING	A9IVI2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5830214.
GenomeReviews	Gene locus BT_1358 in contig AM260525_GR.
PATRIC	20550517. VBIBarTri113218_1400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BTRI382640:BT_1358-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BART1

Accession

Primary (citable) accession number: A9IVI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program