A9IVB0 (SYT_BART1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BT_1306

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000077347
Regions
	Region	246 – 549	304	Catalytic HAMAP MF_00184
Sites
	Metal binding	343	1	Zinc; catalytic By similarity
	Metal binding	394	1	Zinc; catalytic By similarity
	Metal binding	526	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IVB0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: A143BCB37A3527D5	FASTA	658	75,537
	10 20 30 40 50 60 MSCSISLSFP DGSKRNYPAE MTGLELAESI SKSLAKKAVA YSLDGITRDL SDSLGQSGQV 70 80 90 100 110 120 EIITREDSRA LELIRHDCAH VLAEAVQELF PETQVTIGPV IENGFYYDFA RQQPFTLDDL 130 140 150 160 170 180 TIIEKKMREI IQRNKPFRKE IWSREKARRV FSEKKEFYKV ELINSIPDNQ DLKIYYQGEW 190 200 210 220 230 240 FDLCRGPHMQ STGQIGNAFK LMKVAGAYWR GDANNPMLTR IYGTAFANEN DLKAYLHMLE 250 260 270 280 290 300 EAEKRDHRRL GREMDLFHFQ EEGPGMIFWH QKGWKMFQNL INYMRRRLDD HQYAEVNAPQ 310 320 330 340 350 360 VLDRSLWEIS GHWGWYKENM FKAIPAAEDL DHEHIYALKP MNCPGHVQIF KHGLKSYRDL 370 380 390 400 410 420 PIRLAEFGLV HRYEPSGSLH GLMRVRSFTQ DDAHIFCTDE QLAAECLSIN DLILSTYADF 430 440 450 460 470 480 GFKEISLKLS TRPEKRVGSD ALWDHAESIM ESVLKTIETK FAGQIKTSIL PGEGAFYGPK 490 500 510 520 530 540 FEYTLKDAIG REWQCGTTQL DFNLPERFGA FYIDKDSEKR QPVMIHRAIF GSMERFLGIL 550 560 570 580 590 600 IENFAGHMPL WLAPEQIVVA TITSEANEYA QKITARLKAV GLSATTDLRN EKINYKIREH 610 620 630 640 650 SLQKVPVILV CGKREAETNS VNMRRLGSPN QTLLSVEDAI KQLSNESTPP DLQRVINA

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIP 105476 / IBS 506.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01670.1.
RefSeq	YP_001609665.1. NC_010161.1.
3D structure databases
ProteinModelPortal	A9IVB0.
SMR	A9IVB0. Positions 245-648.
ModBase	Search...
Protein-protein interaction databases
STRING	A9IVB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5828842.
GenomeReviews	Gene locus BT_1306 in contig AM260525_GR.
PATRIC	20550401. VBIBarTri113218_1346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	VGSDALW.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BTRI382640:BT_1306-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BART1

Accession

Primary (citable) accession number: A9IVB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program