Reviewed, UniProtKB/Swiss-Prot A9IU63 (ALR_BORPD)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Bpet3162

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alanine racemase HAMAP MF_01201		PRO_1000138582
Sites
	Active site	44	1	Proton acceptor; specific for D-alanine By similarity
	Active site	271	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	44	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IU63-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 548CEC69C2969A7E	FASTA	376	39,291
	10 20 30 40 50 60 MPRPIHASIS LAALAHNLDV VRRHLDQAAQ AAGGAPPSIW AVIKANAYGH GIEAAVAGFS 70 80 90 100 110 120 AAQGLAMLDL AEAVRCREAG WGGPILLLEG FFQPADLDLI DRYHLSATVH TREQLDMLAQ 130 140 150 160 170 180 ARLSRRVDIM LKLNSGMNRL GFDPDAYGSA HARALQLREQ GVVGAVGRMT HFACADGTPG 190 200 210 220 230 240 VAGQLRVFQS VTQGLADGPV SVCNSAATLR YPEIAVAHGA QAHWVRPGIC LYGASPFADA 250 260 270 280 290 300 DAASFGLRPA MSLRSQIIGV QDLPAGAEVG YGATFRAERP MRVGVVACGY ADGYPRHAGT 310 320 330 340 350 360 GTPVVVGGVR TRLVGRVSMD MLMVDLDPVP AAGIGTPVSL WGQDGPSVDE VAQAAGTIGY 370 ELLCALAPRV PVKRDS

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP43504.1.
RefSeq	YP_001631772.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5820170.
GenomeReviews	Gene locus Bpet3162 in contig AM902716_GR.
KEGG	bpt:Bpet3162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TGLKPAM.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_BORPD

Accession

Primary (citable) accession number: A9IU63

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)