A9ISG1 (RPOB_BART1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BT_0895

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	1383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1383	1383	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_1000086361

Sequences

	Sequence		Length	Mass (Da)
	A9ISG1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 859A248D8C71B5CA	FASTA	1,383	155,049
	10 20 30 40 50 60 MAQTHAMMSQ FNGRKRVRKF FGKIPEVAEM PNLIEVQKAS YDQFLMIEEP KGGRPDEGLQ 70 80 90 100 110 120 AVFKSVFPIS DFSGTAMLEF VGYEFDLPKF DVEECRQRDL TYAAPLKVIL RLIVFDIDED 130 140 150 160 170 180 TGSKDIKDIK EQGVYMGDMP LMTTNGTFIV NGTERVIVSQ MHRSPGVFFD HDKGKSHSSG 190 200 210 220 230 240 KLLFAARVIP YRGSWLDIEF DAKDIIYARI DRRRKIPVTS LLMALGMDGS DILSTFYDKV 250 260 270 280 290 300 TYERDGEGWR VPYSVDRFKG MKLISDLIDA DSGEVVAEAG KKLTVRTAKS LAEKGLKAVK 310 320 330 340 350 360 VSEDDLLGCY LAEDIVNYET GEIYLEAGDE IDEKVLKILL DVRADQINIL DIDHMNIGAY 370 380 390 400 410 420 IRNTLKVDKN ESRQDALFDI YRVMRPGEPP TIDTAEAMFH SLFFDPERYD LSAVGRVKMN 430 440 450 460 470 480 LRMDLDCPDT VRVLRQEDIL AVVKMLVELR DGRGEIDDID NLGNRRVRSV GELMENQYRI 490 500 510 520 530 540 GLLRMERAIK ERMSSVEIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH 550 560 570 580 590 600 KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF 610 620 630 640 650 660 IESPYRKIID GKVTKEVIYL SAMEEAKHYV AQANSSLDSE GRFTEEFVVC RHAGEVLMAP 670 680 690 700 710 720 RDHVDLMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMEA 730 740 750 760 770 780 VVARDSGAAV SAKRSGIVDQ VDATRIVIRA TEDLDPSKSG VDIYRLQKFQ RSNQSTCINQ 790 800 810 820 830 840 RPLVHVGDRV EKGDIIADGP STDLGDLALG RNVLVAFMPW NGYNYEDSIL LSERIVADDV 850 860 870 880 890 900 FTSIHIEEFE VAARDTKLGP EEITRDIPNV AEEALRNLDE AGIIYIGAEV QPGDILVGKI 910 920 930 940 950 960 TPKGESPMTP EEKLLRAIFG EKASDVRDTS MRMPPGTFGT VVEVRVFNRH GVEKDERAMA 970 980 990 1000 1010 1020 IEREEIERLA KDRDDEQSIL DRNVYARLTD MLTGKVAVEG PKGFSESKKL DSTVMGRYPR 1030 1040 1050 1060 1070 1080 SQWWQFAVED EKLQNEIEAL RKQYDESKEA LQRRFMDKVE KVQRGDEMPP GVMKMVKVFV 1090 1100 1110 1120 1130 1140 AVKRKIQPGD KMAGRHGNKG VVSRILPVED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE 1150 1160 1170 1180 1190 1200 THLGWACAGM GKKIGDLLEL YQETGDILPL RQRIENLMPD DNHNEPVRQY DNESLYKLAL 1210 1220 1230 1240 1250 1260 QMKKGVSIAT PVFDGAHESD INMMLEDAGL DSSGQVTLYD GRTGEPFDRP VTVGYIYMLK 1270 1280 1290 1300 1310 1320 LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK 1330 1340 1350 1360 1370 1380 SDDVAGRTKV YEAIVRGDDT FEAGIPESFN VLVKEMRSLA LNVELDDARE LIAQRVLSDR VEQ

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIP 105476 / IBS 506.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01298.1.
RefSeq	YP_001609293.1. NC_010161.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9ISG1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5829998.
GenomeReviews	Gene locus BT_0895 in contig AM260525_GR.
PATRIC	20549556. VBIBarTri113218_0932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BTRI382640:BT_0895-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BART1

Accession

Primary (citable) accession number: A9ISG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program