Reviewed, UniProtKB/Swiss-Prot A9IRH6 (SYT_BORPD)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Bpet2834

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000098545
Regions
	Region	247 – 538	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	338	1	Zinc; catalytic By similarity
	Metal binding	389	1	Zinc; catalytic By similarity
	Metal binding	515	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IRH6-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0ACABD26091B19A7	FASTA	650	73,361
	10 20 30 40 50 60 MVQITLPDGS QRQFPGPVTV AEVAQSIGTG LAKAALGGRV TEPGGEPRLV DTSYRLEHDA 70 80 90 100 110 120 QLAIVTAKDA DGLDMIRHST AHLLAYAVKE LFPDAQVTIG PVIDNGFYYD FSYKRPFTPE 130 140 150 160 170 180 DLAAIEKKMT ELARKDETVT REEWTRDDAV AYFKSIGEDY KAEIIASIPA NETLSLYREG 190 200 210 220 230 240 NFVDLCRGPH VPSTGKLKVF KLMKVAGAYW RGDSNNEMLQ RIYGTAWATK DDQDAYLHML 250 260 270 280 290 300 EEAERRDHRK IGRDLDLFHF QDEAPGLIFW HPKGWALWQQ VEQYMRAVYN DNGYQEVKAP 310 320 330 340 350 360 QILDLSLWKK TGHWDNYREN MFTTESENRV YGLKPMNCPG HVQIFNAGLH SYRELPLRYG 370 380 390 400 410 420 EFGQCHRNEP SGSLHGMMRV RGFTQDDGHI FCTEDQLQDE CADFTALLQK VYRDFGFHEV 430 440 450 460 470 480 LYKVATRPEK RIGSDDIWDK AETALMESLR RTGCEFEISP GEGAFYGPKI EYTLKDAIGR 490 500 510 520 530 540 HWQCGTVQVD FSMPVRLGAE YVDAQDQRRA PVMLHRAILG SLERFIGMLI ENHAGAMPPW 550 560 570 580 590 600 LAPVQAVVCC ISEPSADYAA QITQTLKKQG FRVQSDLRGE KITRKIREHS LQKVPYILVV 610 620 630 640 650 GDKEKQNGTV AVRGLGGLDL GVIALEDFAA RLAEDVAARR DVVQPESSAN

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP43176.1.
RefSeq	YP_001631444.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5817395.
GenomeReviews	Gene locus Bpet2834 in contig AM902716_GR.
KEGG	bpt:Bpet2834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NTKIAIG.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BORPD

Accession

Primary (citable) accession number: A9IRH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)