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A9IRC2

- SYI_BORPD

UniProt

A9IRC2 - SYI_BORPD

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Bpet2814
Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi916 – 9161Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBPET340100:GJBO-2847-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Bpet2814
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001225: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligaseUniRule annotationPRO_1000189130Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet2814.

Structurei

3D structure databases

ProteinModelPortaliA9IRC2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9IRC2-1 [UniParc]FASTAAdd to Basket

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MDYKKTLNLP DTPFPMRGDL AKREPAWVAQ WEENHVYQAI RAASRGRPRF    50
VLHDGPPYAN GDIHIGHAVN KVLKDIIVKS RNMAGYDAHY VPGWDCHGMP 100
IEIQIEKKFG KHLPVTEVQA KARAYALEQI DRQRKDFKRL GVLGEWDRPY 150
LTMNFSNEAN EIRALGGILQ KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD 200
RVDPAVDVAF PFADKPGLAA AFGLDSVDDG AVVIWTTTPW TIPANQALNM 250
HPELEYALVR ATPAPAHGPL LLLARERVEA CLKAWGLQGE VIATAPGAAL 300
SGLRFHHPLA SADAGYARTS PVYLGDYVTL DAGTGVVHSA PAYGIEDFVS 350
CKAHGLSDDD ILNPVLGDGK YADSLPLFGG LSIWDANPRI IEALKAAGSL 400
MDVQKLSHSY MHCWRHKTPI IYRATSQWFA GMDVKPADGG PTLRESALAG 450
IDATAFYPSW GRARLHAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGALHP 500
RTAELLEQVA QRVEQHGIEA WQSLDPRELL GDEADQYEKN RDTLDVWFDS 550
GTTHATVLGG KDQALGGSHG AELAWPADLY LEGSDQHRGW FHSSLLTGCM 600
LYGQPPYKAL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV 650
ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANVADF DAVSQAVPYG 700
ELFEIDRYAL TMTAQMQAEV LAHYERYDFH PAVSRLQTFC SEDLGAFYLD 750
ILKDRLYTTA AGSLARRSAQ TALLDITQAL LKLMAPILSF TAEEAWQVLA 800
QSALQHQADV SRTTIFTEVY HALPPFADAD ALAAKWARLR AIRAEVQRKL 850
EDVRTAGGIG SSLQAEVDLY AGGDDRALLA SLGDDLRFVL IVSRATVHEA 900
QGELRVEITP SAHKKCERCW HWRLDVGSDA DHPEICGRCV TNLFGAGEPR 950
DKA 953
Length:953
Mass (Da):105,198
Last modified:February 5, 2008 - v1
Checksum:iA994E669957A6EF7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1.
RefSeqiWP_012249722.1. NC_010170.1.
YP_001631424.1. NC_010170.1.

Genome annotation databases

EnsemblBacteriaiCAP43156; CAP43156; Bpet2814.
GeneIDi5817009.
KEGGibpt:Bpet2814.
PATRICi21167146. VBIBorPet31633_2829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1 .
RefSeqi WP_012249722.1. NC_010170.1.
YP_001631424.1. NC_010170.1.

3D structure databases

ProteinModelPortali A9IRC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340100.Bpet2814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP43156 ; CAP43156 ; Bpet2814 .
GeneIDi 5817009.
KEGGi bpt:Bpet2814.
PATRICi 21167146. VBIBorPet31633_2829.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BPET340100:GJBO-2847-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Entry informationi

Entry nameiSYI_BORPD
AccessioniPrimary (citable) accession number: A9IRC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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