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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei582Aminoacyl-adenylateUniRule annotation1
Binding sitei626ATPUniRule annotation1
Metal bindingi916ZincUniRule annotation1
Metal bindingi919ZincUniRule annotation1
Metal bindingi936ZincUniRule annotation1
Metal bindingi939ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bpet2814
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001225 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891301 – 953Isoleucine--tRNA ligaseAdd BLAST953

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet2814.

Structurei

3D structure databases

ProteinModelPortaliA9IRC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi623 – 627"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9IRC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKKTLNLP DTPFPMRGDL AKREPAWVAQ WEENHVYQAI RAASRGRPRF
60 70 80 90 100
VLHDGPPYAN GDIHIGHAVN KVLKDIIVKS RNMAGYDAHY VPGWDCHGMP
110 120 130 140 150
IEIQIEKKFG KHLPVTEVQA KARAYALEQI DRQRKDFKRL GVLGEWDRPY
160 170 180 190 200
LTMNFSNEAN EIRALGGILQ KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD
210 220 230 240 250
RVDPAVDVAF PFADKPGLAA AFGLDSVDDG AVVIWTTTPW TIPANQALNM
260 270 280 290 300
HPELEYALVR ATPAPAHGPL LLLARERVEA CLKAWGLQGE VIATAPGAAL
310 320 330 340 350
SGLRFHHPLA SADAGYARTS PVYLGDYVTL DAGTGVVHSA PAYGIEDFVS
360 370 380 390 400
CKAHGLSDDD ILNPVLGDGK YADSLPLFGG LSIWDANPRI IEALKAAGSL
410 420 430 440 450
MDVQKLSHSY MHCWRHKTPI IYRATSQWFA GMDVKPADGG PTLRESALAG
460 470 480 490 500
IDATAFYPSW GRARLHAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGALHP
510 520 530 540 550
RTAELLEQVA QRVEQHGIEA WQSLDPRELL GDEADQYEKN RDTLDVWFDS
560 570 580 590 600
GTTHATVLGG KDQALGGSHG AELAWPADLY LEGSDQHRGW FHSSLLTGCM
610 620 630 640 650
LYGQPPYKAL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV
660 670 680 690 700
ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANVADF DAVSQAVPYG
710 720 730 740 750
ELFEIDRYAL TMTAQMQAEV LAHYERYDFH PAVSRLQTFC SEDLGAFYLD
760 770 780 790 800
ILKDRLYTTA AGSLARRSAQ TALLDITQAL LKLMAPILSF TAEEAWQVLA
810 820 830 840 850
QSALQHQADV SRTTIFTEVY HALPPFADAD ALAAKWARLR AIRAEVQRKL
860 870 880 890 900
EDVRTAGGIG SSLQAEVDLY AGGDDRALLA SLGDDLRFVL IVSRATVHEA
910 920 930 940 950
QGELRVEITP SAHKKCERCW HWRLDVGSDA DHPEICGRCV TNLFGAGEPR

DKA
Length:953
Mass (Da):105,198
Last modified:February 5, 2008 - v1
Checksum:iA994E669957A6EF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1.

Genome annotation databases

EnsemblBacteriaiCAP43156; CAP43156; Bpet2814.
KEGGibpt:Bpet2814.
PATRICi21167146. VBIBorPet31633_2829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1.

3D structure databases

ProteinModelPortaliA9IRC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340100.Bpet2814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP43156; CAP43156; Bpet2814.
KEGGibpt:Bpet2814.
PATRICi21167146. VBIBorPet31633_2829.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_BORPD
AccessioniPrimary (citable) accession number: A9IRC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.