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A9IRC2

- SYI_BORPD

UniProt

A9IRC2 - SYI_BORPD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
Binding sitei626 – 6261ATPUniRule annotation
Metal bindingi916 – 9161ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBPET340100:GJBO-2847-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bpet2814
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001225: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligasePRO_1000189130Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet2814.

Structurei

3D structure databases

ProteinModelPortaliA9IRC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9IRC2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKKTLNLP DTPFPMRGDL AKREPAWVAQ WEENHVYQAI RAASRGRPRF
60 70 80 90 100
VLHDGPPYAN GDIHIGHAVN KVLKDIIVKS RNMAGYDAHY VPGWDCHGMP
110 120 130 140 150
IEIQIEKKFG KHLPVTEVQA KARAYALEQI DRQRKDFKRL GVLGEWDRPY
160 170 180 190 200
LTMNFSNEAN EIRALGGILQ KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD
210 220 230 240 250
RVDPAVDVAF PFADKPGLAA AFGLDSVDDG AVVIWTTTPW TIPANQALNM
260 270 280 290 300
HPELEYALVR ATPAPAHGPL LLLARERVEA CLKAWGLQGE VIATAPGAAL
310 320 330 340 350
SGLRFHHPLA SADAGYARTS PVYLGDYVTL DAGTGVVHSA PAYGIEDFVS
360 370 380 390 400
CKAHGLSDDD ILNPVLGDGK YADSLPLFGG LSIWDANPRI IEALKAAGSL
410 420 430 440 450
MDVQKLSHSY MHCWRHKTPI IYRATSQWFA GMDVKPADGG PTLRESALAG
460 470 480 490 500
IDATAFYPSW GRARLHAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGALHP
510 520 530 540 550
RTAELLEQVA QRVEQHGIEA WQSLDPRELL GDEADQYEKN RDTLDVWFDS
560 570 580 590 600
GTTHATVLGG KDQALGGSHG AELAWPADLY LEGSDQHRGW FHSSLLTGCM
610 620 630 640 650
LYGQPPYKAL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV
660 670 680 690 700
ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANVADF DAVSQAVPYG
710 720 730 740 750
ELFEIDRYAL TMTAQMQAEV LAHYERYDFH PAVSRLQTFC SEDLGAFYLD
760 770 780 790 800
ILKDRLYTTA AGSLARRSAQ TALLDITQAL LKLMAPILSF TAEEAWQVLA
810 820 830 840 850
QSALQHQADV SRTTIFTEVY HALPPFADAD ALAAKWARLR AIRAEVQRKL
860 870 880 890 900
EDVRTAGGIG SSLQAEVDLY AGGDDRALLA SLGDDLRFVL IVSRATVHEA
910 920 930 940 950
QGELRVEITP SAHKKCERCW HWRLDVGSDA DHPEICGRCV TNLFGAGEPR

DKA
Length:953
Mass (Da):105,198
Last modified:February 5, 2008 - v1
Checksum:iA994E669957A6EF7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1.
RefSeqiWP_012249722.1. NC_010170.1.
YP_001631424.1. NC_010170.1.

Genome annotation databases

EnsemblBacteriaiCAP43156; CAP43156; Bpet2814.
GeneIDi5817009.
KEGGibpt:Bpet2814.
PATRICi21167146. VBIBorPet31633_2829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP43156.1 .
RefSeqi WP_012249722.1. NC_010170.1.
YP_001631424.1. NC_010170.1.

3D structure databases

ProteinModelPortali A9IRC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340100.Bpet2814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP43156 ; CAP43156 ; Bpet2814 .
GeneIDi 5817009.
KEGGi bpt:Bpet2814.
PATRICi 21167146. VBIBorPet31633_2829.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BPET340100:GJBO-2847-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Entry informationi

Entry nameiSYI_BORPD
AccessioniPrimary (citable) accession number: A9IRC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3