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Protein

UvrABC system protein B

Gene

uvrB

Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 55ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:Bpet2780
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001225 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000995391 – 675UvrABC system protein BAdd BLAST675

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet2780.

Structurei

3D structure databases

ProteinModelPortaliA9IR19.
SMRiA9IR19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 192Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini439 – 605Helicase C-terminalUniRule annotationAdd BLAST167
Domaini633 – 668UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 124Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiTEYHQMI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

A9IR19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPGFVDFP DSPFHLYQPY PPAGDQPAAI EGLAQGMRDG LMYQTLLGVT
60 70 80 90 100
GSGKTYTMAN IIARLGRPAL VLAPNKTLAA QLYAEMREFF PKNAVEYFVS
110 120 130 140 150
YYDYYQPEAY VPTRDLFIEK DSSINEHIEQ MRLSATKSLL ERRDTVIVGT
160 170 180 190 200
VSCIYGIGNP GDYHAMVLIL RAGDRISRRE VLARLVAMQY TRNDADFARG
210 220 230 240 250
TFRVRGETLD IFPAESPELA LRLTLFDDEI ESLELFDPLT GRVRQKVPRF
260 270 280 290 300
TVYPGSHYVT PRDTVLRAIE TIKEELRDRL KLLTAEGKLV EAQRLEQRTR
310 320 330 340 350
FDLEMLQELG FCKGIENYSR HLSGAAPGEP PPTLIDYLPA DALMFIDESH
360 370 380 390 400
VTMGQLGGMY RGDRARKETL VQYGFRLPSA LDNRPLRLEE FEARMRQCVF
410 420 430 440 450
VSATPAAYEQ EHSDNVVEQV VRPTGLVDPQ VEVRPARTQV DDLLGEIKLR
460 470 480 490 500
VAAQERVLVT TLTKRMAEDL TDFLAEHGVR VRYLHSDIDT VERVEIIRDL
510 520 530 540 550
RLGTFDVLVG INLLREGLDI PEVSLVAILD ADKEGFLRSE RSLIQTIGRA
560 570 580 590 600
ARNLNGHAIL YADAITASMR RAMDETERRR TKQLAFNAEH GITARGVNKA
610 620 630 640 650
VRELIDGIVA PARHDTLESA IAPEVLADEK SVAREIRRLE KLMTDHARNL
660 670
EFEQAAAARD ALNALKQRVL LDGAA
Length:675
Mass (Da):75,938
Last modified:February 5, 2008 - v1
Checksum:i0CC38986C598EAF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM902716 Genomic DNA. Translation: CAP43122.1.

Genome annotation databases

EnsemblBacteriaiCAP43122; CAP43122; Bpet2780.
KEGGibpt:Bpet2780.

Similar proteinsi

Entry informationi

Entry nameiUVRB_BORPD
AccessioniPrimary (citable) accession number: A9IR19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 5, 2008
Last modified: October 25, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families