Reviewed, UniProtKB/Swiss-Prot A9IQ81 (PYRD_BART1)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BT_0576

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000336456
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IQ81-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8A403EC0D4EF371F	FASTA	360	39,905
	10 20 30 40 50 60 MSFFRCIGRS ALFMLDPEHA HRLAIVGLKS GLNGCQKVVD KRLSVTIAGL KFKNFIGLAA 70 80 90 100 110 120 GFDKNAEVVD EIFRLGFGFT EIGTVTPKPQ IGNPKPRLFR LKEDEAIINR MGFNNDGHQV 130 140 150 160 170 180 VDDRLRICKK AGVVGVNIGA NKDTVDKIDD YTVGIAHFYD VADYFTVNIS SPNTPGLRDL 190 200 210 220 230 240 QARDSLHLLL NAISKARKEQ EKKHGFSIPI FLKIAPDLSE KELDDIAEEI KLSDFDGLIV 250 260 270 280 290 300 SNTTLSRQGL NNSHFSNEEG GLSGRPLFER STIVLAKMRQ KLGKEIAIIG VGGVKDAQTA 310 320 330 340 350 360 LEKVKAGADL IQLYSGMVYE GPNLVITILK EILQMMQQDG VDNIKDYRDH NLEHWAKFPL

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK01023.1.
RefSeq	YP_001609018.1.
3D structure databases
SMR	A9IQ81. Positions 4-332.
ModBase	Search...
Genome annotation databases
GeneID	5829298.
GenomeReviews	Gene locus BT_0576 in contig AM260525_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351027.
OMA	AALNRMG.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BART1

Accession

Primary (citable) accession number: A9IQ81

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)