Reviewed, UniProtKB/Swiss-Prot A9IP11 (GCP_BORPD)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	Bpet2551

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000145951
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A9IP11-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 1782F2827F71E4CA	FASTA	348	36,196
	10 20 30 40 50 60 MIILGFESSC DETGVAAVST ERGLLAHALH TQIAMHQEYG GVVPELASRD HIRRAVPLAR 70 80 90 100 110 120 QVLAEAGLGL QDVDAVAYTA GPGLAGALLV GASVAQAFAW ARGLPAIPIH HLEGHLLSPL 130 140 150 160 170 180 LDDPRPDFPF VALLVSGGHT QLMRVDGVGR YALLGETLDD AAGEAFDKSA KLMGLGYPGG 190 200 210 220 230 240 PALSRLAASG DPRRYALPRP MLHSGDLDFS FSGLKTAVLT RVKEAERDGG LDDAARADLA 250 260 270 280 290 300 AATQAAIVEV LVAKAVRALK QTGLKRLVVA GGVGANQLLR ELLAQALRPL GARAYFPPLA 310 320 330 340 LCTDNGAMIA FAAAERVKAG LASLQAGQHA FTVRPRWDLA DICAGTPA

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP42893.1.
RefSeq	YP_001631161.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	5816748.
GenomeReviews	Gene locus Bpet2551 in contig AM902716_GR.
KEGG	bpt:Bpet2551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BORPD

Accession

Primary (citable) accession number: A9IP11

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)