A9ILS3 (ILVD_BART1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BT_0106

Organism

Bartonella tribocorum (strain CIP 105476 / IBS 506) [Complete proteome] [HAMAP]

Taxonomic identifier

382640 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073966
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A9ILS3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 69E808619F28B169	FASTA	612	65,846
	10 20 30 40 50 60 MPSYRSRIST HGRNMAGARG LWRATGMKDA DFGKPIIAIA NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AQQIEIAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSREII ADSVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPT IFVSGGPMEA GKIKWKDQDL RVDLVDAMIA AASEHNSEEE 190 200 210 220 230 240 VAEMERAACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS VLATHRDRRM LFEEAGRQIV 250 260 270 280 290 300 ALVKRYYEKD DETVLPRSIA SRKAFENAMT VDIAMGGSTN TVLHLLAAAQ EGEVDFTMTD 310 320 330 340 350 360 IDHLSRRVPV LCKVAPAVAN VHMEDVHRAG GIMGLLGELD AAGLIDTSTY TVHAKTMKEA 370 380 390 400 410 420 LDHWDIKQTN EPTVCAFYRA APGGIPTQRA FSQSCRYETL DLDRAKGVIR DKEHAYSQDG 430 440 450 460 470 480 GLAVLYGNLA KDGCIVKTAG VDQSILTFKG PARIFESQDS AVSAILNDKI KTGEIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKVCAL VTDGRFSGGS SGLSIGHVSP EAAEGGEIAL 550 560 570 580 590 600 VEEGDIIEID IPNRSIHMLV DDVEMKQRRA KMAAKGNGAW QPIEKRQRKV SKALKAYAAM 610 TTSAAKGAVR NI

References

[1]

"Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors." Saenz H.L., Engel P., Stoeckli M.C., Lanz C., Raddatz G., Vayssier-Taussat M., Birtles R., Schuster S.C., Dehio C. Nat. Genet. 39:1469-1476(2007) [PubMed: 18037886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIP 105476 / IBS 506.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260525 Genomic DNA. Translation: CAK00596.1.
RefSeq	YP_001608591.1. NC_010161.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9ILS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5830573.
GenomeReviews	Gene locus BT_0106 in contig AM260525_GR.
PATRIC	20547928. VBIBarTri113218_0128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BTRI382640:BT_0106-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BART1

Accession

Primary (citable) accession number: A9ILS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program