Reviewed, UniProtKB/Swiss-Prot A9IKD8 (METE_BORPD)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	Bpet2059

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097816
Sites
	Metal binding	652	1	Zinc By similarity
	Metal binding	654	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IKD8-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: ABEB0EE99AE81F48	FASTA	764	84,426
	10 20 30 40 50 60 MTITHNLGFP RIGAQRELKR AVEAYWSGKK TAADLEETGR ELRAAHWQRQ AASGLQWVPV 70 80 90 100 110 120 GDFAWYDHIL EWTTLLGAVP ARFGQPEGQP VSLDTLFRMG RGRAPSGTPT AACEMTKWFD 130 140 150 160 170 180 TNYHYIVPEL VPGQTFRIAR EYLFEQVQEA QALGHQVKPV IPGPLTWLWQ GKGDAYAGGA 190 200 210 220 230 240 SDAAKLQLLD TLLPVYAEVL ARLARLGVEW VQIDEPILVL DLPQAWRDAF RTAYARLADA 250 260 270 280 290 300 PVKLLVATYF GGLNDNLATA LALPVAGLHV DLIRAPGQLQ DVAAGLRPGQ VLSAGIIDGR 310 320 330 340 350 360 NIWRTDLDAA LAALAPARQL LGERLWLAPS CSLLHVPVDL ANETDLDAEL RSWLSFAAQK 370 380 390 400 410 420 LDELGLLGRA LADARAPGVD EALAAQRAAL RARRQSARIH NPAVGRRMAE SNAVTRERAP 430 440 450 460 470 480 FAERIARQQQ LLKLPAFPTT TIGSFPQTAE IRALRRDWKS GALSDSAYEA AIRKEIESVI 490 500 510 520 530 540 RFQEKIGLDV LVHGEPERND MVEYFGELLA GFAFTRNGWV QSYGSRCVKP PIIFGDVARP 550 560 570 580 590 600 APMTVGWSSY AQSLTDKPVK GMLTGPVTIL QWSFVRDDQP REQTCRQLAL ALRDEVVDLE 610 620 630 640 650 660 QAGVRVIQID EPAIREGLPL RRADWQAYLD WAVDCFRLST AGVAADTQIH THMCYSEFND 670 680 690 700 710 720 IIESIAAMDA DVITIETSRS NMELLKAFED FRYPNDIGPG VYDIHSPNVP DVDWMVGLMR 730 740 750 760 KAAGRLPSER LWVNPDCGLK TRAWPETEAA LVGMVEAARA LRAG

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP42399.1.
RefSeq	YP_001630668.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5820386.
GenomeReviews	Gene locus Bpet2059 in contig AM902716_GR.
KEGG	bpt:Bpet2059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BORPD

Accession

Primary (citable) accession number: A9IKD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)