Reviewed, UniProtKB/Swiss-Prot A9IIK3 (AMPA_BORPD)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bpet1771

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098305
Sites
	Active site	283	1	Potential
	Active site	357	1	Potential
	Metal binding	271	1	Manganese 2 By similarity
	Metal binding	276	1	Manganese 1 By similarity
	Metal binding	276	1	Manganese 2 By similarity
	Metal binding	294	1	Manganese 2 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 1 By similarity
	Metal binding	355	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IIK3-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E60F1C92059125D7	FASTA	498	52,271
	10 20 30 40 50 60 MEFSTQSTAS LHQIKTAALA VGVYADGELS PAADVIDRAS NNAVRQVVKA EFRGRPGATL 70 80 90 100 110 120 VLRALPGVSA QRVVLVGLGK QSEYNARAHA AAEQGFAAAC VAARITEAVS TLAANSIADT 130 140 150 160 170 180 PIRARARSAA IAAGAATYHY DATFGKPDRD ARPKLKKIVQ VVERGEAAQT QQGLREGSAI 190 200 210 220 230 240 AHGMELTRTL GNLPGNVCTP TYLGDTARKL AREFKSLKVE VLDRKQVEAL GMGSFLSVAR 250 260 270 280 290 300 GSDEPLRFIV LRHAGKPAKK SKAGPVVLVG KGITFDAGGI SLKPAATMDE MKYDMCGAAS 310 320 330 340 350 360 VLGTFRALAE LELPLDVVGL IAACENLPSG KANKPGDIVT SMSGQTIEIL NTDAEGRLVL 370 380 390 400 410 420 CDALTYAERF KPSAVVDIAT LTGACVVALG HVNTGLFTQD DALADALLAA GRQSLDTAWR 430 440 450 460 470 480 MPMDDAYQDQ LKSNFADVAN IGGPPAGSVT AACFLSRFAK AYRWAHLDIA GTAWRSGKDK 490 GATGRPVPLL MQFLLDQA

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP42111.1.
RefSeq	YP_001630379.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	5817352.
GenomeReviews	Gene locus Bpet1771 in contig AM902716_GR.
KEGG	bpt:Bpet1771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LGHHNSG.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BORPD

Accession

Primary (citable) accession number: A9IIK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)