A9II44 (ISPD_BORPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Bpet1695

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000094311
Sites
	Site	17	1	Transition state stabilizer By similarity
	Site	25	1	Transition state stabilizer By similarity
	Site	154	1	Positions MEP for the nucleophilic attack By similarity
	Site	210	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9II44 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: B763EB9841D5980A	FASTA	224	23,029
	10 20 30 40 50 60 MTDSLIAIVP AAGVGQRAGR PDLPKQYRLL AGQPMLRWAV AALLADARIG QVRVAVSPGD 70 80 90 100 110 120 ERAGAALAGL PRTVCRPCGG PTRAATVAAA LADSNAADSD WILVHDAARP GLPPDALARL 130 140 150 160 170 180 IDACLADAVG GLLALPVADT VKAGGPRVRA TLDRDGLWLA QTPQMFRAGV LRAALAAAQA 190 200 210 220 GGGTVTDEAS AIEAAGHAPL LVPGAMRNFK VTWPDDFELM EKWL

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM902716 Genomic DNA. Translation: CAP42034.1.
RefSeq	YP_001630303.1. NC_010170.1.
3D structure databases
ProteinModelPortal	A9II44.
ModBase	Search...
Protein-protein interaction databases
STRING	A9II44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5819449.
GenomeReviews	Gene locus Bpet1695 in contig AM902716_GR.
KEGG	bpt:Bpet1695.
PATRIC	21164837. VBIBorPet31633_1683.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	BPET94624:BPET1695-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_BORPD

Accession

Primary (citable) accession number: A9II44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program