A9IHH4 (MASZ_BORPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Bpet4866

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella petrii

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Malate synthase G HAMAP-Rule MF_00641		PRO_1000130887
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9IHH4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9248C75C6A1258C5	FASTA	726	78,962
	10 20 30 40 50 60 MTERIQQDGL QVAAVLHRFI QDEALPAGGI DAPRFWAGFA ALVRDLAPRN RALLAERDRL 70 80 90 100 110 120 QRELDTWHRA HPGPVRDLRA YRAFLEQIGY LQPVPGTVRV ETANVDAEIA EQAGPQLVVP 130 140 150 160 170 180 MSNARYALNA ANARWGSLYD ALYGTDAIPP TPGDTGRGYH PQRGQAVIAR ARAFLDEAVP 190 200 210 220 230 240 LAQGSHADAT GYSVDNGQLR VALAQGSTGL AQPEQFAGYQ GDAQAPQAIL LKHHGLHIEI 250 260 270 280 290 300 QIDRAHSIGA TDAAGIKDVV VEAALTTIMD CEDSVAAVDA EDKVQVYRNW LGLMKGDLAE 310 320 330 340 350 360 QVTKGGQTFT RRLNADRVYH APGGGTLTLH GRSLMFVRNV GHLMTNPAVL DADGNEIPEG 370 380 390 400 410 420 ILDAVVTTLA ALPDRASRRN SRAGSIYIVK PKMHGPAEAA FANELFDRVE DLLGLPRHTV 430 440 450 460 470 480 KMGIMDEERR TSVNLKACIQ AAAGRVAFIN TGFLDRTGDE MHSSMEAGPM MRKGDMKSSA 490 500 510 520 530 540 WIAAYERSNV LVGLDCGLRG RAQIGKGMWA MPDLMAAMLE QKIGHPKAGA NTAWVPSPTA 550 560 570 580 590 600 ATLHALHYHQ VDVPAVQQQL ESTRLASVQD ELLDGLLTVP VGNPADWSPD DIRHELENNA 610 620 630 640 650 660 QGILGYVVRW IDQGVGCSKV PDINNVGLME DRATLRISSQ HIANWMRHGI ATREQVRDTF 670 680 690 700 710 720 ERMAAVVDRQ NAGDPLYQPM AGHFDTSIAF QAACALVFEG LAQPNGYTEP LLHQYRLAFK ARHNKG

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM902716 Genomic DNA. Translation: CAP45218.1.
RefSeq	YP_001633485.1. NC_010170.1.
3D structure databases
ProteinModelPortal	A9IHH4.
SMR	A9IHH4. Positions 2-722.
ModBase	Search...
Protein-protein interaction databases
STRING	340100.Bpet4866.
Proteomic databases
PRIDE	A9IHH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP45218; CAP45218; Bpet4866.
GeneID	5819865.
KEGG	bpt:Bpet4866.
PATRIC	21171336. VBIBorPet31633_4894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	SQFIENE.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	BPET340100:GJBO-4937-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BORPD

Accession

Primary (citable) accession number: A9IHH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program