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A9IFG1 (PROB_BORPD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:Bpet4680
OrganismBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]
Taxonomic identifier340100 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000125214

Regions

Domain286 – 36479PUA
Nucleotide binding179 – 1802ATP By similarity
Nucleotide binding221 – 2277ATP By similarity

Sites

Binding site201ATP By similarity
Binding site601Substrate By similarity
Binding site1471Substrate By similarity
Binding site1591Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A9IFG1 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: B00B18FE3003F756

FASTA37840,246
        10         20         30         40         50         60 
MTADTTAVSV VASATRLVAK VGSSLVTNEG RGLDRAAVAH WAAQIAALRQ QGKQVVLVSS 

        70         80         90        100        110        120 
GAIAEGMARL GWRKRPSVMH ELQAAAAVGQ MGLCQAYEAA FAEHGLRTAQ ILLTHEDLAD 

       130        140        150        160        170        180 
RHRYLNARST LFALMRLGVV PIVNENDTVV TDEIRLGDND TLGALVTNLI EADALVILTD 

       190        200        210        220        230        240 
QRGLYDSDPR KNPDAVFVAH AQAGDPELEA MAGGAGSGIG TGGMLTKILA AKRAAHSGAH 

       250        260        270        280        290        300 
TVIASGRERN VLTRLAQGEC IGTELRAVLP VWSARKQWLA DHLRLRGRVV LDAGAVRALL 

       310        320        330        340        350        360 
HEGKSLLPIG VIDVQGEFER GDVVACIDPQ GAECARGLIN YSSADTRRIL RQPSSQIARI 

       370 
LGSMTDPELM HRDNLVVI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM902716 Genomic DNA. Translation: CAP45031.1.
RefSeqYP_001633298.1. NC_010170.1.

3D structure databases

ProteinModelPortalA9IFG1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340100.Bpet4680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP45031; CAP45031; Bpet4680.
GeneID5818761.
KEGGbpt:Bpet4680.
PATRIC21170951. VBIBorPet31633_4702.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycBPET340100:GJBO-4750-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_BORPD
AccessionPrimary (citable) accession number: A9IFG1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways