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A9I7M5

- NAPA_BORPD

UniProt

A9I7M5 - NAPA_BORPD

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Protein

Periplasmic nitrate reductase

Gene
napA, Bpet4079
Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBPET340100:GJBO-4142-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Bpet4079
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001225: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed predictionAdd
BLAST
Chaini32 – 847816Periplasmic nitrate reductaseUniRule annotationPRO_1000186350Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet4079.

Structurei

3D structure databases

ProteinModelPortaliA9I7M5.
SMRiA9I7M5. Positions 40-846.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCACKVER.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9I7M5-1 [UniParc]FASTAAdd to Basket

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MSLNRREFIK TNAVAAAAAT AGMSLPAAAL AQAGSPPLQV RWDKAPCRFC    50
GTGCSVLVGV QNDRVVATQG DPEAPVNRGL NCIKGYFLSK IMYGADRLTR 100
PMLRKKNGAY DKNGEFTPIS WDEAFDIMAQ KWKQALAAGG PDAIAMFGSG 150
QWTIWEGYAA SKLYKAGFRS NNLDPNARHC MASAVVGFMR TFGIDEPMGC 200
YDDIEHADAF VLWGSNMAEM HPILWSRITD RRLTHAGSEV HVLSTFEHRS 250
FELADNGMIF VPQTDLAILN YLCNYLIQNG RVNREFVEQH VNFKVGATDI 300
GYGLRPNDPR EQSATSNGYP GADGKPKGDP GAATPATFEE FAAFVSTYTL 350
DYTARLSGVP AEKLQRMAEL YADPDKKIVS FWTMGFNQHA RGTWVNNMIY 400
NVHLLTGKIS QPGCGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVGNPAH 450
RKIAEELWQL PEGTIPAKVG LHAVAQSRAL KDGKLKCYWT STTNNMQAGP 500
NINDEIYPGW RNPEAFVVVS DPYPTVSALS ADLILPTAMW VEKEGAYGNA 550
ERRTQFWRQQ VRAPGESRSD LWQYLEFAKR FKIEEVWPAE LLDKKPEYRG 600
KTLYDVLYAN GVVNRYPLAD VGVVNKHAID GYMNDESQHF GFYVQKGLFE 650
EYAQFGRGHA HDLADFDLYH RARGLRWPVV DGKETLWRFR EGYDPYVKQG 700
EGVRFYGNKD GKANIFALPY QPPAEVPDQD YDLWLCTGRV LEHWHTGSMT 750
RRVPELHRAV PEAVLFMHPD DAKERGLQRG MQCKIASRRG EVFASLETRG 800
RNKPPRGLVY MPFFDEGRLV NKLTLDATCP LSKETDFKKC AVKVQKA 847
Length:847
Mass (Da):94,698
Last modified:February 5, 2008 - v1
Checksum:iFFBCA8BEE235F1E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP44427.1.
RefSeqiWP_012250968.1. NC_010170.1.
YP_001632695.1. NC_010170.1.

Genome annotation databases

EnsemblBacteriaiCAP44427; CAP44427; Bpet4079.
GeneIDi5816252.
KEGGibpt:Bpet4079.
PATRICi21169795. VBIBorPet31633_4127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP44427.1 .
RefSeqi WP_012250968.1. NC_010170.1.
YP_001632695.1. NC_010170.1.

3D structure databases

ProteinModelPortali A9I7M5.
SMRi A9I7M5. Positions 40-846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340100.Bpet4079.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP44427 ; CAP44427 ; Bpet4079 .
GeneIDi 5816252.
KEGGi bpt:Bpet4079.
PATRICi 21169795. VBIBorPet31633_4127.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi CACKVER.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BPET340100:GJBO-4142-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Entry informationi

Entry nameiNAPA_BORPD
AccessioniPrimary (citable) accession number: A9I7M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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