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A9I7M5 (NAPA_BORPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Bpet4079
OrganismBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]
Taxonomic identifier340100 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 847816Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186350

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9I7M5 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: FFBCA8BEE235F1E2

FASTA84794,698
        10         20         30         40         50         60 
MSLNRREFIK TNAVAAAAAT AGMSLPAAAL AQAGSPPLQV RWDKAPCRFC GTGCSVLVGV 

        70         80         90        100        110        120 
QNDRVVATQG DPEAPVNRGL NCIKGYFLSK IMYGADRLTR PMLRKKNGAY DKNGEFTPIS 

       130        140        150        160        170        180 
WDEAFDIMAQ KWKQALAAGG PDAIAMFGSG QWTIWEGYAA SKLYKAGFRS NNLDPNARHC 

       190        200        210        220        230        240 
MASAVVGFMR TFGIDEPMGC YDDIEHADAF VLWGSNMAEM HPILWSRITD RRLTHAGSEV 

       250        260        270        280        290        300 
HVLSTFEHRS FELADNGMIF VPQTDLAILN YLCNYLIQNG RVNREFVEQH VNFKVGATDI 

       310        320        330        340        350        360 
GYGLRPNDPR EQSATSNGYP GADGKPKGDP GAATPATFEE FAAFVSTYTL DYTARLSGVP 

       370        380        390        400        410        420 
AEKLQRMAEL YADPDKKIVS FWTMGFNQHA RGTWVNNMIY NVHLLTGKIS QPGCGPFSLT 

       430        440        450        460        470        480 
GQPSACGTAR EVGTFAHRLP ADMVVGNPAH RKIAEELWQL PEGTIPAKVG LHAVAQSRAL 

       490        500        510        520        530        540 
KDGKLKCYWT STTNNMQAGP NINDEIYPGW RNPEAFVVVS DPYPTVSALS ADLILPTAMW 

       550        560        570        580        590        600 
VEKEGAYGNA ERRTQFWRQQ VRAPGESRSD LWQYLEFAKR FKIEEVWPAE LLDKKPEYRG 

       610        620        630        640        650        660 
KTLYDVLYAN GVVNRYPLAD VGVVNKHAID GYMNDESQHF GFYVQKGLFE EYAQFGRGHA 

       670        680        690        700        710        720 
HDLADFDLYH RARGLRWPVV DGKETLWRFR EGYDPYVKQG EGVRFYGNKD GKANIFALPY 

       730        740        750        760        770        780 
QPPAEVPDQD YDLWLCTGRV LEHWHTGSMT RRVPELHRAV PEAVLFMHPD DAKERGLQRG 

       790        800        810        820        830        840 
MQCKIASRRG EVFASLETRG RNKPPRGLVY MPFFDEGRLV NKLTLDATCP LSKETDFKKC 


AVKVQKA 

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References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM902716 Genomic DNA. Translation: CAP44427.1.
RefSeqYP_001632695.1. NC_010170.1.

3D structure databases

ProteinModelPortalA9I7M5.
SMRA9I7M5. Positions 40-846.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340100.Bpet4079.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP44427; CAP44427; Bpet4079.
GeneID5816252.
KEGGbpt:Bpet4079.
PATRIC21169795. VBIBorPet31633_4127.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADAFGFYI.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycBPET340100:GJBO-4142-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BORPD
AccessionPrimary (citable) accession number: A9I7M5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families