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A9I7M5

- NAPA_BORPD

UniProt

A9I7M5 - NAPA_BORPD

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBPET340100:GJBO-4142-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Bpet4079
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001225: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 847816Periplasmic nitrate reductasePRO_1000186350Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet4079.

Structurei

3D structure databases

ProteinModelPortaliA9I7M5.
SMRiA9I7M5. Positions 40-846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCACKVER.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9I7M5-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSLNRREFIK TNAVAAAAAT AGMSLPAAAL AQAGSPPLQV RWDKAPCRFC
60 70 80 90 100
GTGCSVLVGV QNDRVVATQG DPEAPVNRGL NCIKGYFLSK IMYGADRLTR
110 120 130 140 150
PMLRKKNGAY DKNGEFTPIS WDEAFDIMAQ KWKQALAAGG PDAIAMFGSG
160 170 180 190 200
QWTIWEGYAA SKLYKAGFRS NNLDPNARHC MASAVVGFMR TFGIDEPMGC
210 220 230 240 250
YDDIEHADAF VLWGSNMAEM HPILWSRITD RRLTHAGSEV HVLSTFEHRS
260 270 280 290 300
FELADNGMIF VPQTDLAILN YLCNYLIQNG RVNREFVEQH VNFKVGATDI
310 320 330 340 350
GYGLRPNDPR EQSATSNGYP GADGKPKGDP GAATPATFEE FAAFVSTYTL
360 370 380 390 400
DYTARLSGVP AEKLQRMAEL YADPDKKIVS FWTMGFNQHA RGTWVNNMIY
410 420 430 440 450
NVHLLTGKIS QPGCGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVGNPAH
460 470 480 490 500
RKIAEELWQL PEGTIPAKVG LHAVAQSRAL KDGKLKCYWT STTNNMQAGP
510 520 530 540 550
NINDEIYPGW RNPEAFVVVS DPYPTVSALS ADLILPTAMW VEKEGAYGNA
560 570 580 590 600
ERRTQFWRQQ VRAPGESRSD LWQYLEFAKR FKIEEVWPAE LLDKKPEYRG
610 620 630 640 650
KTLYDVLYAN GVVNRYPLAD VGVVNKHAID GYMNDESQHF GFYVQKGLFE
660 670 680 690 700
EYAQFGRGHA HDLADFDLYH RARGLRWPVV DGKETLWRFR EGYDPYVKQG
710 720 730 740 750
EGVRFYGNKD GKANIFALPY QPPAEVPDQD YDLWLCTGRV LEHWHTGSMT
760 770 780 790 800
RRVPELHRAV PEAVLFMHPD DAKERGLQRG MQCKIASRRG EVFASLETRG
810 820 830 840
RNKPPRGLVY MPFFDEGRLV NKLTLDATCP LSKETDFKKC AVKVQKA
Length:847
Mass (Da):94,698
Last modified:February 5, 2008 - v1
Checksum:iFFBCA8BEE235F1E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP44427.1.
RefSeqiWP_012250968.1. NC_010170.1.
YP_001632695.1. NC_010170.1.

Genome annotation databases

EnsemblBacteriaiCAP44427; CAP44427; Bpet4079.
GeneIDi5816252.
KEGGibpt:Bpet4079.
PATRICi21169795. VBIBorPet31633_4127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM902716 Genomic DNA. Translation: CAP44427.1 .
RefSeqi WP_012250968.1. NC_010170.1.
YP_001632695.1. NC_010170.1.

3D structure databases

ProteinModelPortali A9I7M5.
SMRi A9I7M5. Positions 40-846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340100.Bpet4079.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP44427 ; CAP44427 ; Bpet4079 .
GeneIDi 5816252.
KEGGi bpt:Bpet4079.
PATRICi 21169795. VBIBorPet31633_4127.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi CACKVER.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BPET340100:GJBO-4142-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Entry informationi

Entry nameiNAPA_BORPD
AccessioniPrimary (citable) accession number: A9I7M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3