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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei84Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei151Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei384Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei388Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei494Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei543Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei570Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei813Substrate; via amide nitrogenUniRule annotation1
Binding sitei821Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei838Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Bpet4079
OrganismiBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)
Taxonomic identifieri340100 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001225 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_100018635032 – 847Periplasmic nitrate reductaseUniRule annotationAdd BLAST816

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiA9I7M5

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi340100.Bpet4079

Structurei

3D structure databases

ProteinModelPortaliA9I7M5
SMRiA9I7M5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 964Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 220Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni244 – 248Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni263 – 265Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni520 – 521Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni737 – 746Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9I7M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLNRREFIK TNAVAAAAAT AGMSLPAAAL AQAGSPPLQV RWDKAPCRFC
60 70 80 90 100
GTGCSVLVGV QNDRVVATQG DPEAPVNRGL NCIKGYFLSK IMYGADRLTR
110 120 130 140 150
PMLRKKNGAY DKNGEFTPIS WDEAFDIMAQ KWKQALAAGG PDAIAMFGSG
160 170 180 190 200
QWTIWEGYAA SKLYKAGFRS NNLDPNARHC MASAVVGFMR TFGIDEPMGC
210 220 230 240 250
YDDIEHADAF VLWGSNMAEM HPILWSRITD RRLTHAGSEV HVLSTFEHRS
260 270 280 290 300
FELADNGMIF VPQTDLAILN YLCNYLIQNG RVNREFVEQH VNFKVGATDI
310 320 330 340 350
GYGLRPNDPR EQSATSNGYP GADGKPKGDP GAATPATFEE FAAFVSTYTL
360 370 380 390 400
DYTARLSGVP AEKLQRMAEL YADPDKKIVS FWTMGFNQHA RGTWVNNMIY
410 420 430 440 450
NVHLLTGKIS QPGCGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVGNPAH
460 470 480 490 500
RKIAEELWQL PEGTIPAKVG LHAVAQSRAL KDGKLKCYWT STTNNMQAGP
510 520 530 540 550
NINDEIYPGW RNPEAFVVVS DPYPTVSALS ADLILPTAMW VEKEGAYGNA
560 570 580 590 600
ERRTQFWRQQ VRAPGESRSD LWQYLEFAKR FKIEEVWPAE LLDKKPEYRG
610 620 630 640 650
KTLYDVLYAN GVVNRYPLAD VGVVNKHAID GYMNDESQHF GFYVQKGLFE
660 670 680 690 700
EYAQFGRGHA HDLADFDLYH RARGLRWPVV DGKETLWRFR EGYDPYVKQG
710 720 730 740 750
EGVRFYGNKD GKANIFALPY QPPAEVPDQD YDLWLCTGRV LEHWHTGSMT
760 770 780 790 800
RRVPELHRAV PEAVLFMHPD DAKERGLQRG MQCKIASRRG EVFASLETRG
810 820 830 840
RNKPPRGLVY MPFFDEGRLV NKLTLDATCP LSKETDFKKC AVKVQKA
Length:847
Mass (Da):94,698
Last modified:February 5, 2008 - v1
Checksum:iFFBCA8BEE235F1E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM902716 Genomic DNA Translation: CAP44427.1

Genome annotation databases

EnsemblBacteriaiCAP44427; CAP44427; Bpet4079
KEGGibpt:Bpet4079

Similar proteinsi

Entry informationi

Entry nameiNAPA_BORPD
AccessioniPrimary (citable) accession number: A9I7M5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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