A9I7L6 (GCST_BORPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	Bpet4076

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Aminomethyltransferase HAMAP MF_00259		PRO_1000114079

Sequences

	Sequence		Length	Mass (Da)
	A9I7L6 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8CEA55B91F82A7B5	FASTA	366	39,484
	10 20 30 40 50 60 MSASLKRTPL ADAHVAAGAR MVDFGGWDMP LAYGSQLEEH HAVRRDAGMF DVSHMLNVDV 70 80 90 100 110 120 TGPDATAFLR RLVANDVARL TVPGKALYSC MLNPQGGIID DLIVYFFAAD QWRVVVNAAT 130 140 150 160 170 180 ADKDVAWMQR VAAAGSYDVA IAPRRDLAMV AVQGPNARAR VWAARPAWQA ATEPLTPFVA 190 200 210 220 230 240 AQVADDTLVA RTGYTGEDGF EIVLPADQVV ALWNDLLAQG VRPCGLGARD TLRLEAGMNL 250 260 270 280 290 300 YGQDMDELTQ PDQAGLTWTV SLKDPERRFV GRDALEQFAA PRGFVGLKLQ ERGVMRAHMA 310 320 330 340 350 360 VYTPLGEGEI TSGTMSPTLG VSVAFARVPQ GVQPGQTVEV DIRGKRVPAL VCKLPFVRHG KAVEHS

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM902716 Genomic DNA. Translation: CAP44424.1.
RefSeq	YP_001632692.1. NC_010170.1.
3D structure databases
ProteinModelPortal	A9I7L6.
SMR	A9I7L6. Positions 7-364.
ModBase	Search...
Protein-protein interaction databases
STRING	A9I7L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5819852.
GenomeReviews	Gene locus Bpet4076 in contig AM902716_GR.
KEGG	bpt:Bpet4076.
PATRIC	21169787. VBIBorPet31633_4123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	BPET94624:BPET4076-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BORPD

Accession

Primary (citable) accession number: A9I7L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program