A9I4V6 (MURC_BORPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Bpet0698

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336816
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A9I4V6 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 94CD1D7FEEB16C17	FASTA	468	48,986
	10 20 30 40 50 60 MKHRIQHIHF VGIGGSGMSG IAEVLLNLGY TISGSDLQES AVTRRLAGLG AHVAIGHTAA 70 80 90 100 110 120 NVAGAGAIVT STAVAGDNPE VIAARAAGIP VVPRAIMLAE LMRLKRGIAV AGTHGKTTTT 130 140 150 160 170 180 SLVASVLAAG DLDPTFVIGG RLTSAGANAR LGQGEYIVVE ADESDASFLN LLPVMAIVTN 190 200 210 220 230 240 IDADHMDTYG HDVARLKSAF IEFTQRLPFY GSAVLCADDA NVREIMPFVS RPITTYGLSP 250 260 270 280 290 300 DAHVRGENVQ ADGTRMRFTV QRQDRATALP PLAIELNLPG LHNVRNALAA VAVATELGVP 310 320 330 340 350 360 DEAIAQALAS FKGVGRRFTQ TGEFPVPAAH GGGTFTLIDD YGHHPVEMAA TLAAARGAWP 370 380 390 400 410 420 DRRIVLAFQP HRYTRTRDCF EDFVRVLGGA DAVLLTEVYA AGEPPLVAAD GRALARALRV 430 440 450 460 AGRIEPVFVE DVADLPQAVL DFVRDGDVVV VMGAGSISKV PGLVGELA

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-461 / DSM 12804 / CCUG 43448.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM902716 Genomic DNA. Translation: CAP41030.1.
RefSeq	YP_001629301.1. NC_010170.1.
3D structure databases
ProteinModelPortal	A9I4V6.
SMR	A9I4V6. Positions 3-464.
ModBase	Search...
Protein-protein interaction databases
STRING	A9I4V6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5819508.
GenomeReviews	Gene locus Bpet0698 in contig AM902716_GR.
KEGG	bpt:Bpet0698.
PATRIC	21162863. VBIBorPet31633_0704.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BPET94624:BPET0698-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BORPD

Accession

Primary (citable) accession number: A9I4V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program