Reviewed, UniProtKB/Swiss-Prot A9I0G7 (PYRE_BORPD)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	Bpet0423

Organism

Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]

Taxonomic identifier

340100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138765
Regions
	Region	37 – 38	2	Orotate binding By similarity
	Region	75 – 76	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	130 – 138	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	29	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	105	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	106	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	109	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	111	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	134	1	Orotate By similarity
	Binding site	162	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9I0G7-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8C7424C10635B827	FASTA	225	23,392
	10 20 30 40 50 60 MAAASATSTD FVRFALDEGV LRFGSFKVKS GRTSPYFFNA GLFNSGASVG RLAQFYAQAL 70 80 90 100 110 120 VDSGIAFDML FGPAYKGIPL ATATAVALAG HPAMQGRDVP FAFNRKEAKD HGEGGTLVGA 130 140 150 160 170 180 PLQGRVVIID DVITAGTSVR ESVEIIRAAG AEPAAVLIAL DRQERAGPDD ALSPHSAVQD 190 200 210 220 VAKTYGMPVV SIASLADILA LLQGDAQFAG NREAVLAYRG KYGVV

References

[1]

"The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae." Gross R., Guzman C.A., Sebaihia M., Martin dos Santos V.A.P., Pieper D.H., Koebnik R., Lechner M., Bartels D., Buhrmester J., Choudhuri J.V., Ebensen T., Gaigalat L., Herrmann S., Khachane A.N., Larisch C., Link S., Linke B., Meyer F. , Mormann S., Nakunst D., Rueckert C., Schneiker-Bekel S., Schulze K., Voerholter F.-J., Yevsa T., Engle J.T., Goldman W.E., Puehler A., Goebel U.B., Goesmann A., Bloecker H., Kaiser O., Martinez-Arias R. BMC Genomics 9:449-449(2008) [PubMed: 18826580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM902716 Genomic DNA. Translation: CAP40755.1.
RefSeq	YP_001629026.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5818649.
GenomeReviews	Gene locus Bpet0423 in contig AM902716_GR.
KEGG	bpt:Bpet0423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YKGITLA.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BORPD

Accession

Primary (citable) accession number: A9I0G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)