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A9HZV6 (CYSG_BORPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Siroheme synthase

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:cysG
Ordered Locus Names:Bpet3643
OrganismBordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) [Complete proteome] [HAMAP]
Taxonomic identifier340100 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP-Rule MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP-Rule MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP-Rule MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP-Rule MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP-Rule MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

Belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Siroheme synthase HAMAP-Rule MF_01646
PRO_1000186935

Regions

Region218 – 461244Uroporphyrinogen-III C-methyltransferase HAMAP-Rule MF_01646

Sequences

Sequence LengthMass (Da)Tools
A9HZV6 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 7CF77890DA635545

FASTA47451,002
        10         20         30         40         50         60 
MKLFPLFADL QGRRVLVVGG GEIAARKVQL LLEASADVRV GAPALTPELA LLAAQGRIHA 

        70         80         90        100        110        120 
LQGEFRPDWL DDAWLVVAAT DDRAVNAAVS QAAQARRIFS NVVDDAELSS FQVPSIVDRS 

       130        140        150        160        170        180 
PLVVAISSSG VAPVLARRLR EKVESLFDHS LGALAALAAR HRPRIRERRP DLKQRRKFYD 

       190        200        210        220        230        240 
WLLDGPVAAL LRQQRPEEAE RLLETALQAP QDDGAGSVVL VGAGPGDPGL LTLKALRALN 

       250        260        270        280        290        300 
EADVILYDRL VSPAVLALAR RDADRVAVGK RPGEDHDATQ ARIHALLVEH ARAGRRVVRL 

       310        320        330        340        350        360 
KGGDAFIFGR GGEELEYLRA HGVRYEVVPG ITAALACAAY AGIPLTHRDH AQSVRLVTAH 

       370        380        390        400        410        420 
CREDEDTLDW AGLAREHQTL AFYMGVGQLE TLSARLIAHG RSGATPFALI ENGSRPEQRV 

       430        440        450        460        470 
VSGRLADLAA VARSHGVRAP ALLIVGEVAG LARELHWFGA HLEGQRPAPA ALAA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM902716 Genomic DNA. Translation: CAP43986.1.
RefSeqYP_001632254.1. NC_010170.1.

3D structure databases

ProteinModelPortalA9HZV6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340100.Bpet3643.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP43986; CAP43986; Bpet3643.
GeneID5820298.
KEGGbpt:Bpet3643.
PATRIC21168903. VBIBorPet31633_3685.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0007.
HOGENOMHOG000290518.
KOK02302.
OMADAWYAIA.
OrthoDBEOG6DRPFR.
ProtClustDBCLSK881271.

Enzyme and pathway databases

BioCycBPET340100:GJBO-3697-MONOMER.
UniPathwayUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3D1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01646. Siroheme_synth.
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. SSF53790. 1 hit.
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG_BORPD
AccessionPrimary (citable) accession number: A9HZV6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways