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A9HS61 (TRMD_GLUDA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:GDI3089, Gdia_3278
OrganismGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP]
Taxonomic identifier272568 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter ›

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_1000082521

Regions

Region132 – 1376S-adenosyl-L-methionine binding By similarity

Sites

Binding site1121S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A9HS61 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 23F4311C80111A0B

FASTA23725,634
        10         20         30         40         50         60 
MTWRATVLTL FPDMFPGPLG QSLAGRALEG GLWSVEARNL REYGLGRHRV VDDTPFGGGA 

        70         80         90        100        110        120 
GMVMRPDVVS AAIRDVAPAG RPLVYPTPRG RPLRQDDVRR YAGGPGLVVL CGRYEGVDER 

       130        140        150        160        170        180 
ALEACGAEQV SIGDYVLSGG EMAALVLLDS CVRLIPGVMG SDASGTEESF SDGLLEYPHY 

       190        200        210        220        230 
TKPASWEGRD VPDILLSGHH AAIAGWRQTE SETATRTRRP DLWAAHLERT GCTDRQF

« Hide

References

[1]"Gluconacetobacter diazotrophicus Pal5 complete genome."
Bertalan M., Baldani I.J., Ferreira P.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49037 / DSM 5601 / PAl5.
[2]"Complete sequence of chromosome of Gluconacetobacter diazotrophicus PAl 5."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Triplett E.W.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49037 / DSM 5601 / PAl5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM889285 Genomic DNA. Translation: CAP57032.1.
CP001189 Genomic DNA. Translation: ACI53005.1.
RefSeqYP_001603321.1. NC_010125.1.
YP_002277620.1. NC_011365.1.

3D structure databases

ProteinModelPortalA9HS61.
ModBaseSearch...

Protein-protein interaction databases

STRING272568.GDI_3089.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI53005; ACI53005; Gdia_3278.
CAP57032; CAP57032; GDI3089.
GeneID5791918.
6976717.
KEGGgdi:GDI_3089.
gdj:Gdia_3278.
PATRIC22054986. VBIGluDia203729_3262.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHOG000016242.
KOK00554.
OMAPRLLMSP.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycGDIA272568:GJPS-3143-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_GLUDA
AccessionPrimary (citable) accession number: A9HS61
Secondary accession number(s): B5ZL63
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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