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Protein

Biotin synthase

Gene

bioB

Organism
Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi55Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi58Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi96Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi127Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi187Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi259Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:GDI2953, Gdia_3396
OrganismiGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Taxonomic identifieri272568 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter
Proteomesi
  • UP000000736 Componenti: Chromosome
  • UP000001176 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003814091 – 316Biotin synthaseAdd BLAST316

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272568.Gdia_3396.

Structurei

3D structure databases

ProteinModelPortaliA9HRF2.
SMRiA9HRF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PTHR22976:SF32. PTHR22976:SF32. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A9HRF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTDWTRDEI AALIALPFPE LMFRAQSVHR ARFDPTEIQI STLLSIKTGG
60 70 80 90 100
CPEDCAYCPQ SAKHEDGGVK ASRLMAVEAV LKEARAARDA GAARFCMGAA
110 120 130 140 150
WRSPKDHDLE TVCAMVEGVK SLGMETCVTL GMLDDRQAHR LREAGLDYYN
160 170 180 190 200
HNLDTSPEHY GSIISTRTYQ ERLDTLAHVR DAGINVCCGG IVGMGENDDD
210 220 230 240 250
RAGLIASLAS LPRHPESVPI NLLVRVAGTP LAGADPVDPI DFVRIIAAAR
260 270 280 290 300
IAMPASRVRL AAGREDMTDE AQTLCFLAGA NSIFYGEKLL TTPNPEASRD
310
ARLLARLGMH TSLAQA
Length:316
Mass (Da):34,213
Last modified:September 1, 2009 - v2
Checksum:i56B554525102199E
GO

Sequence cautioni

The sequence ACI53122 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAP56896 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM889285 Genomic DNA. Translation: CAP56896.1. Different initiation.
CP001189 Genomic DNA. Translation: ACI53122.1. Different initiation.
RefSeqiWP_012554931.1. NC_011365.1.

Genome annotation databases

EnsemblBacteriaiACI53122; ACI53122; Gdia_3396.
CAP56896; CAP56896; GDI2953.
KEGGigdi:GDI2953.
gdj:Gdia_3396.

Similar proteinsi

Entry informationi

Entry nameiBIOB_GLUDA
AccessioniPrimary (citable) accession number: A9HRF2
Secondary accession number(s): B5ZM22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: August 30, 2017
This is version 69 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families