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A9HRF2 (BIOB_GLUDA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:GDI2953, Gdia_3396
OrganismGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP]
Taxonomic identifier272568 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ACI53122.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAP56896.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Biotin synthase HAMAP-Rule MF_01694
PRO_0000381409

Sites

Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2591Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9HRF2 [UniParc].

Last modified September 1, 2009. Version 2.
Checksum: 56B554525102199E

FASTA31634,213
        10         20         30         40         50         60 
MRTDWTRDEI AALIALPFPE LMFRAQSVHR ARFDPTEIQI STLLSIKTGG CPEDCAYCPQ 

        70         80         90        100        110        120 
SAKHEDGGVK ASRLMAVEAV LKEARAARDA GAARFCMGAA WRSPKDHDLE TVCAMVEGVK 

       130        140        150        160        170        180 
SLGMETCVTL GMLDDRQAHR LREAGLDYYN HNLDTSPEHY GSIISTRTYQ ERLDTLAHVR 

       190        200        210        220        230        240 
DAGINVCCGG IVGMGENDDD RAGLIASLAS LPRHPESVPI NLLVRVAGTP LAGADPVDPI 

       250        260        270        280        290        300 
DFVRIIAAAR IAMPASRVRL AAGREDMTDE AQTLCFLAGA NSIFYGEKLL TTPNPEASRD 

       310 
ARLLARLGMH TSLAQA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM889285 Genomic DNA. Translation: CAP56896.1. Different initiation.
CP001189 Genomic DNA. Translation: ACI53122.1. Different initiation.
RefSeqYP_001603186.1. NC_010125.1.
YP_002277737.1. NC_011365.1.

3D structure databases

ProteinModelPortalA9HRF2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272568.GDI_2953.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI53122; ACI53122; Gdia_3396.
CAP56896; CAP56896; GDI2953.
GeneID5791775.
6976842.
KEGGgdi:GDI_2953.
gdj:Gdia_3396.
PATRIC22055230. VBIGluDia203729_3377.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycGDIA272568:GJPS-3000-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_GLUDA
AccessionPrimary (citable) accession number: A9HRF2
Secondary accession number(s): B5ZM22
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways