A9HLD9 (SYE2_GLUDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	GDI2211, Gdia_0429

Organism

Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP]

Taxonomic identifier

272568 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367676
Regions
	Motif	7 – 17	11	"HIGH" region HAMAP MF_00022_B
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	243	1	ATP By similarity
Experimental info
	Sequence conflict	149 – 153	5	QDRGV → RTVAW in ACI50225. Ref.2
	Sequence conflict	418	1	E → K in ACI50225. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A9HLD9 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: FF25EFA70635693F	FASTA	443	49,680
	10 20 30 40 50 60 MKLRFAPSPT GLIHVGNARQ AIANALYARR HGGTFQLRID DTDRERSRDE YVDALHTDLA 70 80 90 100 110 120 WLGITWDETF RQSDRLDRYA AAIETLKASG RLYPCFESEQ ELASKREARI RMRKPPIYDR 130 140 150 160 170 180 AMLRMTAEQR AQAEANGKVP YWRFRLSDQD RGVDDMVMGR SQVKLQSISD PVLVRADGTV 190 200 210 220 230 240 LYTLASVVDD METGVTHVLR GEDHLTNTGV QIDIAEALGG KVGQFAFAHL PLLLDEAGGK 250 260 270 280 290 300 LSKRFDGLSI RALRQDGIDP VAIVSYLARL GSADDPAPLS FDDLAASYDV RRVSRSAARF 310 320 330 340 350 360 DMRQLLALNR RVMHQMPFGA IRDRLPEGAT EAFWMAVRGN VDMVSELRHW WDVVGGVIVP 370 380 390 400 410 420 PVQDDEGAYL LQALALLPPE PWDAQTWKDW TTAVRDATGR SGKSVFHPLR VALTGEEEGP 430 440 EMRDLLPLMG HDRVAERLRI AAR

References

[1]

"Gluconacetobacter diazotrophicus Pal5 complete genome." Bertalan M., Baldani I.J., Ferreira P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

[2]

"Complete sequence of chromosome of Gluconacetobacter diazotrophicus PAl 5." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Triplett E.W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM889285 Genomic DNA. Translation: CAP56154.1. CP001189 Genomic DNA. Translation: ACI50225.1.
RefSeq	YP_001602456.1. NC_010125.1. YP_002274840.1. NC_011365.1.
3D structure databases
ProteinModelPortal	A9HLD9.
ModBase	Search...
Protein-protein interaction databases
STRING	A9HLD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5792212. 6973823.
GenomeReviews	Gene locus GDI2211 in contig AM889285_GR. Gene locus Gdia_0429 in contig CP001189_GR.
KEGG	gdi:GDI_2211. gdj:Gdia_0429.
PATRIC	22049240. VBIGluDia203729_0436.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_GLUDA

Accession

Primary (citable) accession number: A9HLD9
Secondary accession number(s): B5ZCG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program