A9HKS8 (RNPA_GLUDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	GDI2137, Gdia_0356

Organism

Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP]

Taxonomic identifier

272568 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter › Gluconacetobacter diazotrophicus

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_1000100363

Sequences

	Sequence		Length	Mass (Da)
	A9HKS8 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C7F7D698F0D070D4	FASTA	116	12,878
	10 20 30 40 50 60 MADRAHRLKK RAEFLKVASR GRKVPSPGLV LQALGRDDSD PARIGFTVTK KVGNAVVRNR 70 80 90 100 110 TRRRLREAVR VVEREEPLNG VDLVLIGRDG TRGRTFAALV GDLRRTLRKA GVRGAE

References

[1]

"Gluconacetobacter diazotrophicus Pal5 complete genome." Bertalan M., Baldani I.J., Ferreira P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

[2]

"Complete sequence of chromosome of Gluconacetobacter diazotrophicus PAl 5." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Triplett E.W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001189 Genomic DNA. Translation: ACI50152.1. AM889285 Genomic DNA. Translation: CAP56080.1.
RefSeq	YP_001602382.1. NC_010125.1. YP_002274767.1. NC_011365.1.
3D structure databases
ProteinModelPortal	A9HKS8.
ModBase	Search...
Protein-protein interaction databases
STRING	272568.GDI_2137.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI50152; ACI50152; Gdia_0356. CAP56080; CAP56080; GDI2137.
GeneID	5792496. 6973750.
KEGG	gdi:GDI_2137. gdj:Gdia_0356.
PATRIC	22049094. VBIGluDia203729_0363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG284862.
HOGENOM	HOG000266302.
KO	K03536.
OMA	IRVGFTC.
ProtClustDB	CLSK936517.
Enzyme and pathway databases
BioCyc	GDIA272568-1:GI7A-362-MONOMER. GDIA272568:GJPS-2176-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_GLUDA

Accession

Primary (citable) accession number: A9HKS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program