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A9HJG3

- A9HJG3_GLUDA

UniProt

A9HJG3 - A9HJG3_GLUDA

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511UniRule annotation
Binding sitei54 – 541ATPUniRule annotation
Binding sitei93 – 931ATPUniRule annotation
Metal bindingi95 – 951Magnesium 1UniRule annotation
Active sitei97 – 971Proton acceptorUniRule annotation
Binding sitei118 – 1181SubstrateUniRule annotation
Metal bindingi119 – 1191Magnesium 2UniRule annotation
Binding sitei242 – 2421SubstrateUniRule annotation
Metal bindingi270 – 2701Magnesium 2UniRule annotation
Binding sitei494 – 4941ATPUniRule annotation
Binding sitei531 – 5311ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi532 – 5321Magnesium 1UniRule annotation
Binding sitei534 – 5341SubstrateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGDIA272568:GJPS-1997-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotationImported
Ordered Locus Names:GDI1960Imported
OrganismiGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)Imported
Taxonomic identifieri272568 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter
ProteomesiUP000001176: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA9HJG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 994Substrate bindingUniRule annotation
Regioni314 – 3163Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

KOiK01952.
OMAiRDICGRE.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

A9HJG3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSEKGQRPV DQDLAKEFGL TAEEYGNVLS IMGRTPSLTE LGIFSVMWSE
60 70 80 90 100
HCSYKSSRVW LKTLPTTAPW VIHGPGENAG VVDIGQGLAA IFKMESHNHP
110 120 130 140 150
SFIEPYQGAA TGVGGILRDV FTMGARPVAN LNALRFGSPE NPQTRRIVDG
160 170 180 190 200
VVRGVGGYGN CVGVPTVGGE INFHPSYDGN PLVNAMTVGI ARQDRIFLSA
210 220 230 240 250
AAGVGNPVIY VGSKTGRDGI HGATMSSSEF DEDALAKRPT VQVGDPFVEK
260 270 280 290 300
LLIEACLELM ATDAIVAIQD MGAAGLTSSS VEMAGKGGVG IDLDLDAVPQ
310 320 330 340 350
RERGMTAYEM MLSESQERML IVIRPDRTDV ARAIFEKWEL DFAVIGHLTE
360 370 380 390 400
TGHIVVRHQG RVEADIPLDP LADQAPIYRR PTAPAPVPAP LGPLTDPVGI
410 420 430 440 450
EQALIRLIGC PDLASRAWVW NQYDSTVGGQ TVKRPGGADA AIVKIEDTSL
460 470 480 490 500
GLALTTDCTP RYCRADARLG GAQAVAEAWR NIVATGARPL AVTDNLNFGS
510 520 530 540 550
PEKPEVMGQF VAAIEGMGEA CRALDFPVVS GNVSLYNETR APDGSSVSIL
560 570 580 590 600
PTPAIGGLGV LEDVAKAVGL AMPADCTLVL VGATRGEMGQ SLWLREIHGR
610 620 630 640 650
EDGPPPALDL AAERRNGDFV RGQIQDGHVV ACHDVADGGL LVTLAEMVMA
660 670 680 690 700
GDVGCVLSAP QSGIRPEAFW FGEDQSRYVV AVRDADAFTL AATGAGVETR
710 720 730
VLGRSGGDGL TLPSGATIST ARLNAVNSAF FPALMDR
Length:737
Mass (Da):77,704
Last modified:February 5, 2008 - v1
Checksum:iB2AC151E0A665F73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM889285 Genomic DNA. Translation: CAP55903.1.
RefSeqiWP_012225618.1. NC_010125.1.
YP_001602205.1. NC_010125.1.

Genome annotation databases

EnsemblBacteriaiCAP55903; CAP55903; GDI1960.
GeneIDi5789312.
KEGGigdi:GDI_1960.
PATRICi22059468. VBIGluDia122154_1893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM889285 Genomic DNA. Translation: CAP55903.1 .
RefSeqi WP_012225618.1. NC_010125.1.
YP_001602205.1. NC_010125.1.

3D structure databases

ProteinModelPortali A9HJG3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP55903 ; CAP55903 ; GDI1960 .
GeneIDi 5789312.
KEGGi gdi:GDI_1960.
PATRICi 22059468. VBIGluDia122154_1893.

Phylogenomic databases

KOi K01952.
OMAi RDICGRE.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci GDIA272568:GJPS-1997-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5."
    Bertalan M., Albano R., de Padua V., Rouws L., Rojas C., Hemerly A., Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V., Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.
    , Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F., Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L., Figueiredo D., Montano H., Junior J., de Souza Filho G., Martin Quintana Flores V., Ferreira B., Branco A., Gonzalez P., Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M., Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A., Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T., Rael Pereira A., Silva R., Rondinelli E., von Kruger W., Martins O., Baldani J.I., Ferreira P.C.
    BMC Genomics 10:450-450(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49037 / DSM 5601 / PAl5Imported.

Entry informationi

Entry nameiA9HJG3_GLUDA
AccessioniPrimary (citable) accession number: A9HJG3
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3