Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A9HJG3

- A9HJG3_GLUDA

UniProt

A9HJG3 - A9HJG3_GLUDA

Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei51 – 511UniRule annotation
    Binding sitei54 – 541ATPUniRule annotation
    Binding sitei93 – 931ATPUniRule annotation
    Metal bindingi95 – 951Magnesium 1UniRule annotation
    Active sitei97 – 971Proton acceptorUniRule annotation
    Binding sitei118 – 1181SubstrateUniRule annotation
    Metal bindingi119 – 1191Magnesium 2UniRule annotation
    Binding sitei242 – 2421SubstrateUniRule annotation
    Metal bindingi270 – 2701Magnesium 2UniRule annotation
    Binding sitei494 – 4941ATPUniRule annotation
    Binding sitei531 – 5311ATP; via carbonyl oxygen and amide nitrogenUniRule annotation
    Metal bindingi532 – 5321Magnesium 1UniRule annotation
    Binding sitei534 – 5341SubstrateUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    LigaseUniRule annotation

    Keywords - Biological processi

    Purine biosynthesisUniRule annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciGDIA272568:GJPS-1997-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
    Glutamine amidotransferase PurLUniRule annotation
    Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
    Gene namesi
    Name:purLUniRule annotationImported
    Ordered Locus Names:GDI1960Imported
    OrganismiGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)Imported
    Taxonomic identifieri272568 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter
    ProteomesiUP000001176: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliA9HJG3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni96 – 994Substrate bindingUniRule annotation
    Regioni314 – 3163Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the FGAMS family.UniRule annotation

    Phylogenomic databases

    KOiK01952.
    OMAiRDICGRE.
    OrthoDBiEOG6FNHHR.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.90.650.10. 1 hit.
    HAMAPiMF_00420. PurL_2.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9HJG3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEKGQRPV DQDLAKEFGL TAEEYGNVLS IMGRTPSLTE LGIFSVMWSE    50
    HCSYKSSRVW LKTLPTTAPW VIHGPGENAG VVDIGQGLAA IFKMESHNHP 100
    SFIEPYQGAA TGVGGILRDV FTMGARPVAN LNALRFGSPE NPQTRRIVDG 150
    VVRGVGGYGN CVGVPTVGGE INFHPSYDGN PLVNAMTVGI ARQDRIFLSA 200
    AAGVGNPVIY VGSKTGRDGI HGATMSSSEF DEDALAKRPT VQVGDPFVEK 250
    LLIEACLELM ATDAIVAIQD MGAAGLTSSS VEMAGKGGVG IDLDLDAVPQ 300
    RERGMTAYEM MLSESQERML IVIRPDRTDV ARAIFEKWEL DFAVIGHLTE 350
    TGHIVVRHQG RVEADIPLDP LADQAPIYRR PTAPAPVPAP LGPLTDPVGI 400
    EQALIRLIGC PDLASRAWVW NQYDSTVGGQ TVKRPGGADA AIVKIEDTSL 450
    GLALTTDCTP RYCRADARLG GAQAVAEAWR NIVATGARPL AVTDNLNFGS 500
    PEKPEVMGQF VAAIEGMGEA CRALDFPVVS GNVSLYNETR APDGSSVSIL 550
    PTPAIGGLGV LEDVAKAVGL AMPADCTLVL VGATRGEMGQ SLWLREIHGR 600
    EDGPPPALDL AAERRNGDFV RGQIQDGHVV ACHDVADGGL LVTLAEMVMA 650
    GDVGCVLSAP QSGIRPEAFW FGEDQSRYVV AVRDADAFTL AATGAGVETR 700
    VLGRSGGDGL TLPSGATIST ARLNAVNSAF FPALMDR 737
    Length:737
    Mass (Da):77,704
    Last modified:February 5, 2008 - v1
    Checksum:iB2AC151E0A665F73
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM889285 Genomic DNA. Translation: CAP55903.1.
    RefSeqiYP_001602205.1. NC_010125.1.

    Genome annotation databases

    EnsemblBacteriaiCAP55903; CAP55903; GDI1960.
    GeneIDi5789312.
    KEGGigdi:GDI_1960.
    PATRICi22059468. VBIGluDia122154_1893.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM889285 Genomic DNA. Translation: CAP55903.1 .
    RefSeqi YP_001602205.1. NC_010125.1.

    3D structure databases

    ProteinModelPortali A9HJG3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAP55903 ; CAP55903 ; GDI1960 .
    GeneIDi 5789312.
    KEGGi gdi:GDI_1960.
    PATRICi 22059468. VBIGluDia122154_1893.

    Phylogenomic databases

    KOi K01952.
    OMAi RDICGRE.
    OrthoDBi EOG6FNHHR.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci GDIA272568:GJPS-1997-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.90.650.10. 1 hit.
    HAMAPi MF_00420. PurL_2.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5."
      Bertalan M., Albano R., de Padua V., Rouws L., Rojas C., Hemerly A., Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V., Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.
      , Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F., Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L., Figueiredo D., Montano H., Junior J., de Souza Filho G., Martin Quintana Flores V., Ferreira B., Branco A., Gonzalez P., Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M., Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A., Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T., Rael Pereira A., Silva R., Rondinelli E., von Kruger W., Martins O., Baldani J.I., Ferreira P.C.
      BMC Genomics 10:450-450(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49037 / DSM 5601 / PAl5Imported.

    Entry informationi

    Entry nameiA9HJG3_GLUDA
    AccessioniPrimary (citable) accession number: A9HJG3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 5, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3