Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111ATPUniRule annotation
Binding sitei151 – 1511ATPUniRule annotation
Binding sitei194 – 1941ATPUniRule annotation
Binding sitei217 – 2171ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi156 – 1627ATPUniRule annotation
Nucleotide bindingi186 – 1894ATPUniRule annotation
Nucleotide bindingi271 – 2722ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGDIA272568:GJPS-1756-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:GDI1724Imported, Gdia_3505Imported
OrganismiGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)Imported
Taxonomic identifieri272568 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter
ProteomesiUP000000736 Componenti: Chromosome UP000001176 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272568.GDI_1724.

Structurei

3D structure databases

ProteinModelPortaliA9HHQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 301187ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9HHQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPFPEALP AGAVLGIVGG GQLGRMSAVA AAQLGFRAHI LTDEAEGPAA
60 70 80 90 100
QVAHAVTVGA YDDPDVLRRF ASSVDVVTFE FENISADGLE LLAGLCPVRP
110 120 130 140 150
SGRILRISQD RIAEKRFLAG AGIPLAPWAE IHTPQDLDTA IATLGLPFIL
160 170 180 190 200
KTTRLGYDGK GQHRVHAPDE ALPGFHALAP HPLVAEAMID FACEVSVMVA
210 220 230 240 250
RGLDGTAVTF DVTENRHRNG ILDISLAPAR VSADIAEQAR DIAMRVATAL
260 270 280 290 300
DLVGLLGVEM FVDRDGRVLV NEIAPRPHNS GHWTMDACPV DQFAMHVRAV
310 320 330 340 350
TGLPLPPAVR HSDAVMKNLV GPDDMALWPA ILATPGLLPH HYGKEEARPG
360 370 380
RKMGHVNVLF PHGGLPGEFG VNAALAPCLR NS
Length:382
Mass (Da):40,449
Last modified:February 5, 2008 - v1
Checksum:iADF6FE5E75ADC559
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001189 Genomic DNA. Translation: ACI53229.1.
AM889285 Genomic DNA. Translation: CAP55667.1.
RefSeqiWP_012225199.1. NC_011365.1.
YP_001601969.1. NC_010125.1.
YP_002277844.1. NC_011365.1.

Genome annotation databases

EnsemblBacteriaiACI53229; ACI53229; Gdia_3505.
CAP55667; CAP55667; GDI1724.
KEGGigdi:GDI_1724.
gdj:Gdia_3505.
PATRICi22055462. VBIGluDia203729_3487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001189 Genomic DNA. Translation: ACI53229.1.
AM889285 Genomic DNA. Translation: CAP55667.1.
RefSeqiWP_012225199.1. NC_011365.1.
YP_001601969.1. NC_010125.1.
YP_002277844.1. NC_011365.1.

3D structure databases

ProteinModelPortaliA9HHQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272568.GDI_1724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI53229; ACI53229; Gdia_3505.
CAP55667; CAP55667; GDI1724.
KEGGigdi:GDI_1724.
gdj:Gdia_3505.
PATRICi22055462. VBIGluDia203729_3487.

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.
BioCyciGDIA272568:GJPS-1756-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: PAl 5Imported.
  2. "Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5."
    Bertalan M., Albano R., Padua V., Rouws L., Rojas C., Hemerly A., Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V., Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.
    , Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F., Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L., Figueiredo D., Montano H., Junior J., Filho G., Flores V., Ferreira B., Branco A., Gonzalez P., Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M., Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A., Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T., Kruger W.V., Martins O., Baldani J.I., Ferreira P.C.
    BMC Genomics 10:450-450(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49037 / DSM 5601 / PAl5Imported and PAl 5Imported.
  3. "Two genome sequences of the same bacterial strain, Gluconacetobacter diazotrophicus PAl 5, suggest a new standard in genome sequence submission."
    Giongo A., Tyler H.L., Zipperer U.N., Triplett E.W.
    Stand. Genomic Sci. 2:309-317(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49037 / DSM 5601 / PAl5Imported and PAl 5Imported.

Entry informationi

Entry nameiA9HHQ5_GLUDA
AccessioniPrimary (citable) accession number: A9HHQ5
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: May 27, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.