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A9HF35

- PNP_GLUDA

UniProt

A9HF35 - PNP_GLUDA

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciGDIA272568:GJPS-1394-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:GDI1371, Gdia_2075
    OrganismiGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
    Taxonomic identifieri272568 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter
    ProteomesiUP000000736: Chromosome, UP000001176: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 712712Polyribonucleotide nucleotidyltransferasePRO_0000329665Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272568.GDI_1371.

    Structurei

    3D structure databases

    ProteinModelPortaliA9HF35.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF13014. KH_3. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9HF35-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNYYRKEIE WGGRPLVLET GKIARQADGA VVVTYGDTVV LCTAVGARSV    50
    KPGQDFFPLT VNYQEKAFAA GKIPGGFFKR EGRPSEIEVL NSRLIDRPIR 100
    PLFPENFRNE VQVVATVLSH DLENDPAIAA LIGCSAALTL SGIPFFGPVA 150
    ACRVGYADGA YILNPTLPEM KESALDLVVA GTSEGVLMVE SEASELSEDV 200
    MLGAVTFGHE AFQAVIDAII SLAEHAAKAP WDLAEPSAEE IALKQRIDTL 250
    GRAAIAEAYQ ERVKQQRYKK VGAAKEAVLA ALATEGLDVT AAKPILKDLE 300
    ADVVRSAVLD TGYRIDGRDL KTVRPIVSEV GILPRAHGSA LFTRGETQAL 350
    VVATLGTAQD EQVIDALEGE YRTNFMLHYN FPPYSVGECG RMGSPGRREI 400
    GHGKLAWRAI HPLLPGKDTF PYTMRIVSEI TESNGSSSMA TVCGTSLALM 450
    DAGVPLKRPV AGIAMGLIKE DRGFAVLSDI LGDEDHLGDM DFKVAGTESG 500
    ITALQMDIKI TSITPEIMKI ALGQARDGRL HILGEMSKAL TEGRSDVSST 550
    APKITTISVP KEKIRDVIGQ GGKVIREIVE YSGAKIDIND DGTIMIAASS 600
    EDQATRAIER IRGIVAEPEL GAIYTGKVVK TADFGAFVNF LGARDGLVHI 650
    SELAQGRVAK TTDVVNQGDV VKVKVLGFDD RGKVKLSMRV VDQQTGADIT 700
    ESVGERPGRP AR 712
    Length:712
    Mass (Da):76,309
    Last modified:February 5, 2008 - v1
    Checksum:iDB718FD99A59704E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM889285 Genomic DNA. Translation: CAP55314.1.
    CP001189 Genomic DNA. Translation: ACI51835.1.
    RefSeqiYP_001601627.1. NC_010125.1.
    YP_002276450.1. NC_011365.1.

    Genome annotation databases

    EnsemblBacteriaiACI51835; ACI51835; Gdia_2075.
    CAP55314; CAP55314; GDI1371.
    GeneIDi5792130.
    6975502.
    KEGGigdi:GDI_1371.
    gdj:Gdia_2075.
    PATRICi22052576. VBIGluDia203729_2070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM889285 Genomic DNA. Translation: CAP55314.1 .
    CP001189 Genomic DNA. Translation: ACI51835.1 .
    RefSeqi YP_001601627.1. NC_010125.1.
    YP_002276450.1. NC_011365.1.

    3D structure databases

    ProteinModelPortali A9HF35.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272568.GDI_1371.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI51835 ; ACI51835 ; Gdia_2075 .
    CAP55314 ; CAP55314 ; GDI1371 .
    GeneIDi 5792130.
    6975502.
    KEGGi gdi:GDI_1371.
    gdj:Gdia_2075.
    PATRICi 22052576. VBIGluDia203729_2070.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci GDIA272568:GJPS-1394-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF13014. KH_3. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the sugarcane nitrogen-fixing endophyte Gluconacetobacter diazotrophicus Pal5."
      Bertalan M., Albano R., de Padua V., Rouws L., Rojas C., Hemerly A., Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V., Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.
      , Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F., Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L., Figueiredo D., Montano H., Junior J., de Souza Filho G., Martin Quintana Flores V., Ferreira B., Branco A., Gonzalez P., Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M., Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A., Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T., Rael Pereira A., Silva R., Rondinelli E., von Kruger W., Martins O., Baldani J.I., Ferreira P.C.
      BMC Genomics 10:450-450(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49037 / DSM 5601 / PAl5.
    2. "Two genome sequences of the same bacterial strain, Gluconacetobacter diazotrophicus PAl 5, suggest a new standard in genome sequence submission."
      Giongo A., Tyler H.L., Zipperer U.N., Triplett E.W.
      Stand. Genomic Sci. 2:309-317(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49037 / DSM 5601 / PAl5.

    Entry informationi

    Entry nameiPNP_GLUDA
    AccessioniPrimary (citable) accession number: A9HF35
    Secondary accession number(s): B5ZDK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3