A9HAC0 (ISPE_GLUDA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	GDI0728, Gdia_1283

Organism

Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP]

Taxonomic identifier

272568 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335715
Regions
	Nucleotide binding	111 – 121	11	ATP Potential
Sites
	Active site	17	1	By similarity
	Active site	154	1	By similarity
Experimental info
	Sequence conflict	51	1	R → H in ACI51065. Ref.2
	Sequence conflict	62	1	A → V in ACI51065. Ref.2
	Sequence conflict	139	1	L → P in ACI51065. Ref.2
	Sequence conflict	163	1	A → P in ACI51065. Ref.2
	Sequence conflict	228	1	A → V in ACI51065. Ref.2
	Sequence conflict	278	1	G → A in ACI51065. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A9HAC0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: BF9839CBF565F43E	FASTA	305	31,481
	10 20 30 40 50 60 MTDPTGTGTL HESAHAKINL YLHVTGRRPD GYHLLDSLAV FAGAADRLTL RPGAAGQGEA 70 80 90 100 110 120 VALDIAGAFG AGLVADTDSN LVLQAARRLR AEMGVTDRLA PMRIVLEKSL PVASGIGGGS 130 140 150 160 170 180 ADAAAALRLL LRAWPGEALP RARLMALAVE LGADVPVCID QRAARMGGVG ERLAPAPALP 190 200 210 220 230 240 NCGMMLVNCG EAVPTPAVFR ARAPVFTPAA SLPSAWPDVG AMVRDLAALT NDLQDAACEL 250 260 270 280 290 300 CPTIRTVLQV LDAAPGCRLA RMSGSGATCF ALFDSPQGAR DAMTAVERPG WWVWAGGLHG MPAET

References

[1]

"Gluconacetobacter diazotrophicus Pal5 complete genome." Bertalan M., Baldani I.J., Ferreira P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

[2]

"Complete sequence of chromosome of Gluconacetobacter diazotrophicus PAl 5." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Triplett E.W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM889285 Genomic DNA. Translation: CAP54671.1. CP001189 Genomic DNA. Translation: ACI51065.1.
RefSeq	YP_001601010.1. NC_010125.1. YP_002275680.1. NC_011365.1.
3D structure databases
ProteinModelPortal	A9HAC0.
ModBase	Search...
Protein-protein interaction databases
STRING	A9HAC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5789484. 6974688.
GenomeReviews	Gene locus GDI0728 in contig AM889285_GR. Gene locus Gdia_1283 in contig CP001189_GR.
KEGG	gdi:GDI_0728. gdj:Gdia_1283.
PATRIC	22050944. VBIGluDia203729_1277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	SAHAKIN.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_GLUDA

Accession

Primary (citable) accession number: A9HAC0
Secondary accession number(s): B5ZHI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program