A9GWW2 (A9GWW2_SORC5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193 EMBL CAN93983.1
Ordered Locus Names:	sce3823

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) [Complete proteome] [HAMAP]

Taxonomic identifier

448385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Sorangiineae › Polyangiaceae › Sorangium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL CAN93983.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	38 – 45	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	40	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A9GWW2 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: F44F638F5BC80281	FASTA	324	36,339
	10 20 30 40 50 60 MFSRDVLRID APAVAQEIEQ AIRDQVFGTL RRRGAVVGIS GGIDSSVVST LCVRALGKDK 70 80 90 100 110 120 VLGIFMPERD TSDDALRLGK LLAEGLGIEA VIENIKPALT GLRCYERQDE AIRMVYPDYN 130 140 150 160 170 180 ESWKFKITLP SILESDRLNV SQLTAQPPGE EPRVKRMPLN AYLQLVAATN FKQRIRKMME 190 200 210 220 230 240 YYHADRLNYA VAGTPNLLEY DQGFFVKQGD GAADFKPIAH LYKSQVYALA EYIGVPADIR 250 260 270 280 290 300 RRAPTTDTFS MPQGQDEFYF ALPYDKMDLC LYAYNNNVPA ADAASAVGIT PQQVERVFKD 310 320 IEAKRRVAQY LHAQPLLVRD QGHA

References

[1]

"Complete genome sequence of the myxobacterium Sorangium cellulosum." Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R. Nat. Biotechnol. 25:1281-1289(2007) [PubMed: 17965706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM746676 Genomic DNA. Translation: CAN93983.1.
RefSeq	YP_001614463.1. NC_010162.1.
3D structure databases
ProteinModelPortal	A9GWW2.
ModBase	Search...
Protein-protein interaction databases
STRING	A9GWW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5805291.
GenomeReviews	Gene locus sce3823 in contig AM746676_GR.
KEGG	scl:sce3823.
PATRIC	23665939. VBISorCel80414_4176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A9GWW2_SORC5

Accession

Primary (citable) accession number: A9GWW2

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)