Reviewed, UniProtKB/Swiss-Prot A9FU01 (T23O_SORC5)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	sce8412

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) [Complete proteome] [HAMAP]

Taxonomic identifier

448385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Sorangiineae › Polyangiaceae › Sorangium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Tryptophan 2,3-dioxygenase		PRO_0000360137
Regions
	Region	49 – 53	5	Substrate binding By similarity
	Region	79 – 83	5	Substrate binding By similarity
Sites
	Metal binding	346	1	Iron (heme axial ligand) By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	157	1	Heme By similarity
	Binding site	360	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9FU01-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E7E88A11C43C1370	FASTA	412	47,022
	10 20 30 40 50 60 MRPVPGPSVF WGAGSRLPAP LVARAHRLLR TRRANPARGA SRITKDMNYW DYIHVEELLA 70 80 90 100 110 120 LQGGFDDDEQ KVGNDEALFI VVHQIYELWF KLILRELTSA RELFRQNPVP DQKLASAARS 130 140 150 160 170 180 FRRVVTIFEQ AIEHFRVMET LTTRDYLDFR DRLIPASGFQ SAQLREIELL LGLDDALRIP 190 200 210 220 230 240 LGREGSYKEA LKTADGAPSA ATRRVEARLA SGPSLKHFLD EWLSRTPIDG SSEPEGVVGF 250 260 270 280 290 300 LRSYTRSMRQ ENERRVQMAI DKALTPPDVE RLRARYEAES AAAEAFLLAE DDPAADAATR 310 320 330 340 350 360 EKRRAVRAAI VFIESYRELP RLAWPREVLD RILEMEQAML IWRQRHARMV ERVIGRRTGT 370 380 390 400 410 GGSAGVDYLD QTALRYRVFG DLWTVRSLLL RKPSVPEIEH ASDYGFRVED SP

References

[1]

"Complete genome sequence of the myxobacterium Sorangium cellulosum." Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R. Nat. Biotechnol. 25:1281-1289(2007) [PubMed: 17965706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM746676 Genomic DNA. Translation: CAN98582.1.
RefSeq	YP_001619062.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5812623.
GenomeReviews	Gene locus sce8412 in contig AM746676_GR.
KEGG	scl:sce8412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HFRVMET.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_SORC5

Accession

Primary (citable) accession number: A9FU01

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)