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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Metal bindingi142 – 1421MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSCEL448385:GJ75-5254-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:sce5077
OrganismiSorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56))
Taxonomic identifieri448385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesSorangiineaePolyangiaceaeSorangium
ProteomesiUP000002139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Crossover junction endodeoxyribonuclease RuvCPRO_1000074503Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi448385.sce5077.

Structurei

3D structure databases

ProteinModelPortaliA9FR00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiVGYGIIE.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9FR00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLGIDPGS RHLGWGLLVR QGTRIEHVAH GVIDIDTSGT FAARLVEIDD
60 70 80 90 100
ELGKVIASHA PDAAAVESLF FAKDAQSAAK LGHARGVVLL RLARAGVPIS
110 120 130 140 150
EYPPALVKRT IVGRGAAEKA QVAQVMTAVL RLAAPPRPDA ADALAIAMTH
160 170
LSAAGFAAAL AASGAPVPRR ARPRRARLG
Length:179
Mass (Da):18,575
Last modified:February 5, 2008 - v1
Checksum:i7DAD251BF7439E39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM746676 Genomic DNA. Translation: CAN95240.1.
RefSeqiWP_012237708.1. NC_010162.1.

Genome annotation databases

EnsemblBacteriaiCAN95240; CAN95240; sce5077.
KEGGiscl:sce5077.
PATRICi23668701. VBISorCel80414_5548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM746676 Genomic DNA. Translation: CAN95240.1.
RefSeqiWP_012237708.1. NC_010162.1.

3D structure databases

ProteinModelPortaliA9FR00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi448385.sce5077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAN95240; CAN95240; sce5077.
KEGGiscl:sce5077.
PATRICi23668701. VBISorCel80414_5548.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiVGYGIIE.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciSCEL448385:GJ75-5254-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the myxobacterium Sorangium cellulosum."
    Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A.
    , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R.
    Nat. Biotechnol. 25:1281-1289(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: So ce56.

Entry informationi

Entry nameiRUVC_SORC5
AccessioniPrimary (citable) accession number: A9FR00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: July 22, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.