A9FQE4 (RLME_SORC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase E
EC=2.1.1.166

Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase

Gene names

Name:	rlmE
Synonyms:	ftsJ, rrmJ
Ordered Locus Names:	sce1990

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) [Complete proteome] [HAMAP]

Taxonomic identifier

448385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Myxococcales › Sorangiineae › Polyangiaceae › Sorangium › Sorangium cellulosum

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547
Catalytic activity	S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (uridine-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547		PRO_0000333324
Sites
	Active site	162	1	Proton acceptor By similarity
	Binding site	63	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	65	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	83	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9FQE4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: D96430D39A97E8C0	FASTA	212	22,652
	10 20 30 40 50 60 MPAERPSVSQ KPKNPYKRPD AFTKAAKAQG YPARSVFKLE EIDRRVRLLR PGQRVLDLGA 70 80 90 100 110 120 APGSWSMYAA QRIGAGGKLL AVDLSPITAA FGPQATVVQG DALSLTNEAL AQFAPYDVVL 130 140 150 160 170 180 SDMAPATSGS KIADQARSYE LFMRAVAVAE ALLAPGGAFV GKIFMSEDFV KARDALRNLC 190 200 210 EEVRSIRPEG TRASSVEIFL VGLKRKAAGK TG

References

[1]

"Complete genome sequence of the myxobacterium Sorangium cellulosum." Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R. Nat. Biotechnol. 25:1281-1289(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: So ce56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM746676 Genomic DNA. Translation: CAN92149.1.
RefSeq	YP_001612629.1. NC_010162.1.
3D structure databases
ProteinModelPortal	A9FQE4.
ModBase	Search...
Protein-protein interaction databases
STRING	448385.sce1990.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAN92149; CAN92149; sce1990.
GeneID	5805371.
KEGG	scl:sce1990.
PATRIC	23661881. VBISorCel80414_2165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0293.
HOGENOM	HOG000162366.
KO	K02427.
OMA	NDPYVQR.
Enzyme and pathway databases
BioCyc	SCEL448385:GJ75-2063-MONOMER.
Family and domain databases
HAMAP	MF_01547. RNA_methyltr_E.
InterPro	IPR015507. rRNA-MeTfrase_E. IPR002877. rRNA_MeTrfase_FtsJ_dom. [Graphical view]
PANTHER	PTHR10920. PTHR10920. 1 hit.
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLME_SORC5

Accession

Primary (citable) accession number: A9FQE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program