A9FBU8 (SYM1_SORC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase 1
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase 1
Short name=MetRS 1

Gene names

Name:	metG1
Ordered Locus Names:	sce7883

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) [Complete proteome] [HAMAP]

Taxonomic identifier

448385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Sorangiineae › Polyangiaceae › Sorangium

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Methionine--tRNA ligase 1 HAMAP MF_00098		PRO_0000331919
Regions
	Domain	590 – 690	101	tRNA-binding
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00098
	Motif	328 – 332	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	142	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9FBU8 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 731C7CDE364BA77D	FASTA	690	75,767
	10 20 30 40 50 60 MKRLLLTSAL PYANGHIHIG HLVEYTQTDI FARYWRMTGR RCISLCADDT HGTAIMIRAR 70 80 90 100 110 120 QEGRSEADVI ADMSAAHQRD FAAFQIRFDH YGSTNSPANR ALCEEIWASL RERGMVTTKE 130 140 150 160 170 180 VTQLFDPQEG MFLADRFVKG TCPKCAAPDQ YGDSCDRCGS TYAATDLVEP RSALTGARPE 190 200 210 220 230 240 VRSAQHLMVA IEPERPFLST WTQGEGRMPK EIANYLAGHF LSEPLRDWDV SRPAPYFGFE 250 260 270 280 290 300 IPDAPGNYWY VWFDAPIGYM AASKEWCDRE GEAFDDWWRS EETEIVHVIG KDIVYFHTLF 310 320 330 340 350 360 WPAMLKSARF SLPSRVQVHG FLTVNGEKMS KSKGTFVLAS TYLKHLDPAY LRYYYASKLS 370 380 390 400 410 420 SKVDDIDLNL EELVNKVNAE LVNKVVNLAS RSSRFVAQTG LSAVYPEDGG LFASAAEEGD 430 440 450 460 470 480 AIGAAYAAFD YARATRSIVA LADRANEYVD RMQPWALAKQ PEKKAELQAV CTVALNLFRQ 490 500 510 520 530 540 IVLYLAPVLP KLADDVARLL GCPMDRWALA KTPLVGTPVA AYEHLMKRVE PGAVEKMIAE 550 560 570 580 590 600 GRPAEEAAAP GGAAGANAEA GQAAGGGAAA EDDGAALAKE PLAPECTIDD FSKVDLRVAR 610 620 630 640 650 660 IVAAEHVPEA KKLLKLTVSL GGEARRTVFA GIKAYYKPED LIGRLVIVCA NLAPRKMKFG 670 680 690 LSEGMVLAAG DETVHLLSPD SGAKPGMRVH

References

[1]

"Complete genome sequence of the myxobacterium Sorangium cellulosum." Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R. Nat. Biotechnol. 25:1281-1289(2007) [PubMed: 17965706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: So ce56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM746676 Genomic DNA. Translation: CAN98053.1.
RefSeq	YP_001618533.1. NC_010162.1.
3D structure databases
ProteinModelPortal	A9FBU8.
SMR	A9FBU8. Positions 134-160.
ModBase	Search...
Protein-protein interaction databases
STRING	A9FBU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5812209.
GenomeReviews	Gene locus sce7883 in contig AM746676_GR.
KEGG	scl:sce7883.
PATRIC	23674837. VBISorCel80414_8612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	RASDHAY.
ProtClustDB	PRK00133.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023458. Met-tRNA_synth_1. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit. TIGR00399. MetG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM1_SORC5

Accession

Primary (citable) accession number: A9FBU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program