Reviewed, UniProtKB/Swiss-Prot A9EN94 (ASTD_SORC5)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	sce0676

Organism

Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) [Complete proteome] [HAMAP]

Taxonomic identifier

448385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Sorangiineae › Polyangiaceae › Sorangium

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000085410
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9EN94-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: DBD69EE32F54EACE	FASTA	489	51,883
	10 20 30 40 50 60 MSTHFIDGSW VTGAGEPFAS RNPVTQAVVF EGRAASEEQV DAAVAAARGA FAAWRDRGLE 70 80 90 100 110 120 ERAALVKRFA GVLGEHKARL ADVIGLETGK PRWEALTEVQ TMIGKIDVSL MAWRERTGER 130 140 150 160 170 180 QAEAGDATAV VRHRPHGVVA VLGPYNFPGH LPNGHIVPAL IAGNCVVFKP SELTPRVAEE 190 200 210 220 230 240 TARLWEKAGI PAGVLNLVQG GRRTGVALAG HPGIDGLFFT GSSGTGNALH RQLAGQPEKI 250 260 270 280 290 300 LALEMGGNNP LIVQDVANTE AAIHHILQSS FISAGQRCTC ARRLLVPATP EGDALLGRLV 310 320 330 340 350 360 DAASRLKVGR YDDAEQPFMG AVISLAAADR LLAAQARLVS LGGEVLLEMR RLEEGTALLS 370 380 390 400 410 420 PGILDVSAIK ELPDEEHFGP LVQVQRYGSF DEALSLANRT RYGLAAGLIS DRRELYERFW 430 440 450 460 470 480 RESRAGIVNW NKPLTGASSA APFGGVGSSG NHRPSAYYAA DYCAYPVAGL EAAAVALPGQ LAPGMRLNS

References

[1]

"Complete genome sequence of the myxobacterium Sorangium cellulosum." Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A. , Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H., Puehler A., Mueller R. Nat. Biotechnol. 25:1281-1289(2007) [PubMed: 17965706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM746676 Genomic DNA. Translation: CAN90833.1.
RefSeq	YP_001611313.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5806212.
GenomeReviews	Gene locus sce0676 in contig AM746676_GR.
KEGG	scl:sce0676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A9EN94. EMTQPQA.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_SORC5

Accession

Primary (citable) accession number: A9EN94

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)