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A9CSZ1 (SYEC_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamate--tRNA ligase, cytoplasmic

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
ORF Names:EBI_22577
OrganismEnterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier481877 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaEnterocytozoonidaeEnterocytozoon

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 631631Probable glutamate--tRNA ligase, cytoplasmic
PRO_0000388392

Regions

Nucleotide binding367 – 3715ATP By similarity
Region139 – 1413Glutamate binding By similarity
Region311 – 3155Glutamate binding By similarity
Motif144 – 15310"HIGH" region By similarity
Motif367 – 3715"KMSKS" region By similarity

Sites

Binding site1491ATP By similarity
Binding site1731Glutamate By similarity
Binding site3291Glutamate By similarity
Binding site3321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9CSZ1 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 4DC6852631BEA04D

FASTA63173,962
        10         20         30         40         50         60 
MLENISKSEF VNFLLEKKHN IEPKTLPILE QHIREQKLDD FFLDFSIDFT DEELNNFLSK 

        70         80         90        100        110        120 
LDYWLKNTKL NSSKADVIFG LLYSCNKFIK LIKNPTFSFV EIKQFYNDIL NTNKNYIIEY 

       130        140        150        160        170        180 
NKKDKGKINI AVEGNVVTRF PPEPSGFLHI GHIKAALLND LMAKNGKLLI RFDDTNPIKE 

       190        200        210        220        230        240 
EKMYENVIIE DLHTLGIKNY TIVRSSDHFD SLYNYAIQLI QLGLAYCDNT DQLQMREERT 

       250        260        270        280        290        300 
KGIPSKNRNT DIETNLSIFS KMSSGNCLDY CLRAKIDYTN LNKALRDPVI YRHIEKEHNI 

       310        320        330        340        350        360 
TKNKYKIYPT YDFACPIIDS LDGVTLALRT NEYRDRNEQY YWFLEKLNLP NKPKIYDFSR 

       370        380        390        400        410        420 
LNFENTVLSK RQMKFYVDNH FVSGWDDPRL STLRGILRLG MDIDTLKEYI INQGSSQKSS 

       430        440        450        460        470        480 
VISWDKVWSL NKKNIDHKSA RYSAIPKLYC VECLILDKNN NEIITKTEDI PKFKKNLSLG 

       490        500        510        520        530        540 
NKTIIKSSHI LISQEDANIL NNNEEFTLMN WGNMKVKEKQ IVNGIIIKII LEENLAGDVK 

       550        560        570        580        590        600 
TTKNKLTWVN KENIIEFKIL EYDTLQNDKN TDNLAEKFNT NSKKEEIWLG EKALISVSPK 

       610        620        630 
TYIQIERIGF FICDKPLEFI LIPYTKQKRM R 

« Hide

References

[1]"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi."
Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J.
PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.
[2]"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi."
Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.
PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H348.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ABGB01000003 Genomic DNA. Translation: EDQ31175.1.
RefSeqXP_001828010.1. XM_001827958.1.

3D structure databases

ProteinModelPortalA9CSZ1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6004350.

Organism-specific databases

EuPathDBMicrosporidiaDB:EBI_22577.

Phylogenomic databases

OrthoDBEOG7MKWFN.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYEC_ENTBH
AccessionPrimary (citable) accession number: A9CSZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: February 5, 2008
Last modified: November 13, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries