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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

EBI_22577

Organism
Enterocytozoon bieneusi (strain H348) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149ATPBy similarity1
Binding sitei173GlutamateBy similarity1
Binding sitei329GlutamateBy similarity1
Binding sitei332ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi367 – 371ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
ORF Names:EBI_22577
OrganismiEnterocytozoon bieneusi (strain H348) (Microsporidian parasite)
Taxonomic identifieri481877 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaEnterocytozoonidaeEnterocytozoon
Proteomesi
  • UP000001742 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiMicrosporidiaDB:EBI_22577.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003883921 – 631Probable glutamate--tRNA ligase, cytoplasmicAdd BLAST631

Structurei

3D structure databases

ProteinModelPortaliA9CSZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glutamate bindingBy similarity3
Regioni311 – 315Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi144 – 153"HIGH" regionBy similarity10
Motifi367 – 371"KMSKS" regionBy similarity5

Sequence similaritiesi

Phylogenomic databases

InParanoidiA9CSZ1.
OrthoDBiEOG092C0OTH.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9CSZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLENISKSEF VNFLLEKKHN IEPKTLPILE QHIREQKLDD FFLDFSIDFT
60 70 80 90 100
DEELNNFLSK LDYWLKNTKL NSSKADVIFG LLYSCNKFIK LIKNPTFSFV
110 120 130 140 150
EIKQFYNDIL NTNKNYIIEY NKKDKGKINI AVEGNVVTRF PPEPSGFLHI
160 170 180 190 200
GHIKAALLND LMAKNGKLLI RFDDTNPIKE EKMYENVIIE DLHTLGIKNY
210 220 230 240 250
TIVRSSDHFD SLYNYAIQLI QLGLAYCDNT DQLQMREERT KGIPSKNRNT
260 270 280 290 300
DIETNLSIFS KMSSGNCLDY CLRAKIDYTN LNKALRDPVI YRHIEKEHNI
310 320 330 340 350
TKNKYKIYPT YDFACPIIDS LDGVTLALRT NEYRDRNEQY YWFLEKLNLP
360 370 380 390 400
NKPKIYDFSR LNFENTVLSK RQMKFYVDNH FVSGWDDPRL STLRGILRLG
410 420 430 440 450
MDIDTLKEYI INQGSSQKSS VISWDKVWSL NKKNIDHKSA RYSAIPKLYC
460 470 480 490 500
VECLILDKNN NEIITKTEDI PKFKKNLSLG NKTIIKSSHI LISQEDANIL
510 520 530 540 550
NNNEEFTLMN WGNMKVKEKQ IVNGIIIKII LEENLAGDVK TTKNKLTWVN
560 570 580 590 600
KENIIEFKIL EYDTLQNDKN TDNLAEKFNT NSKKEEIWLG EKALISVSPK
610 620 630
TYIQIERIGF FICDKPLEFI LIPYTKQKRM R
Length:631
Mass (Da):73,962
Last modified:February 5, 2008 - v1
Checksum:i4DC6852631BEA04D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABGB01000003 Genomic DNA. Translation: EDQ31175.1.
RefSeqiXP_001828010.1. XM_001827958.1.

Genome annotation databases

EnsemblFungiiEDQ31175; EDQ31175; EBI_22577.
GeneIDi6004350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABGB01000003 Genomic DNA. Translation: EDQ31175.1.
RefSeqiXP_001828010.1. XM_001827958.1.

3D structure databases

ProteinModelPortaliA9CSZ1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDQ31175; EDQ31175; EBI_22577.
GeneIDi6004350.

Organism-specific databases

EuPathDBiMicrosporidiaDB:EBI_22577.

Phylogenomic databases

InParanoidiA9CSZ1.
OrthoDBiEOG092C0OTH.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYEC_ENTBH
AccessioniPrimary (citable) accession number: A9CSZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: February 5, 2008
Last modified: September 7, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.