A9CSZ1 (SYEC_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

ORF Names:

EBI_22577

Organism

Enterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

481877 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Enterocytozoonidae › Enterocytozoon › Enterocytozoon bieneusi

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Probable glutamate--tRNA ligase, cytoplasmic		PRO_0000388392
Regions
	Nucleotide binding	367 – 371	5	ATP By similarity
	Region	139 – 141	3	Glutamate binding By similarity
	Region	311 – 315	5	Glutamate binding By similarity
	Motif	144 – 153	10	"HIGH" region By similarity
	Motif	367 – 371	5	"KMSKS" region By similarity
Sites
	Binding site	149	1	ATP By similarity
	Binding site	173	1	Glutamate By similarity
	Binding site	329	1	Glutamate By similarity
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9CSZ1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 4DC6852631BEA04D	FASTA	631	73,962
	10 20 30 40 50 60 MLENISKSEF VNFLLEKKHN IEPKTLPILE QHIREQKLDD FFLDFSIDFT DEELNNFLSK 70 80 90 100 110 120 LDYWLKNTKL NSSKADVIFG LLYSCNKFIK LIKNPTFSFV EIKQFYNDIL NTNKNYIIEY 130 140 150 160 170 180 NKKDKGKINI AVEGNVVTRF PPEPSGFLHI GHIKAALLND LMAKNGKLLI RFDDTNPIKE 190 200 210 220 230 240 EKMYENVIIE DLHTLGIKNY TIVRSSDHFD SLYNYAIQLI QLGLAYCDNT DQLQMREERT 250 260 270 280 290 300 KGIPSKNRNT DIETNLSIFS KMSSGNCLDY CLRAKIDYTN LNKALRDPVI YRHIEKEHNI 310 320 330 340 350 360 TKNKYKIYPT YDFACPIIDS LDGVTLALRT NEYRDRNEQY YWFLEKLNLP NKPKIYDFSR 370 380 390 400 410 420 LNFENTVLSK RQMKFYVDNH FVSGWDDPRL STLRGILRLG MDIDTLKEYI INQGSSQKSS 430 440 450 460 470 480 VISWDKVWSL NKKNIDHKSA RYSAIPKLYC VECLILDKNN NEIITKTEDI PKFKKNLSLG 490 500 510 520 530 540 NKTIIKSSHI LISQEDANIL NNNEEFTLMN WGNMKVKEKQ IVNGIIIKII LEENLAGDVK 550 560 570 580 590 600 TTKNKLTWVN KENIIEFKIL EYDTLQNDKN TDNLAEKFNT NSKKEEIWLG EKALISVSPK 610 620 630 TYIQIERIGF FICDKPLEFI LIPYTKQKRM R

References

[1]	"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi." Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J. PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.
[2]	"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi." Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S. PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABGB01000003 Genomic DNA. Translation: EDQ31175.1.
RefSeq	XP_001828010.1. XM_001827958.1.
3D structure databases
ProteinModelPortal	A9CSZ1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6004350.
Organism-specific databases
EuPathDB	MicrosporidiaDB:EBI_22577.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYEC_ENTBH

Accession

Primary (citable) accession number: A9CSZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	February 5, 2008
Last modified:	March 6, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program