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A9CSZ1

- SYEC_ENTBH

UniProt

A9CSZ1 - SYEC_ENTBH

Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

EBI_22577

Organism
Enterocytozoon bieneusi (strain H348) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei149 – 1491ATPBy similarity
    Binding sitei173 – 1731GlutamateBy similarity
    Binding sitei329 – 3291GlutamateBy similarity
    Binding sitei332 – 3321ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi367 – 3715ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. tRNA aminoacylation for protein translation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    ORF Names:EBI_22577
    OrganismiEnterocytozoon bieneusi (strain H348) (Microsporidian parasite)
    Taxonomic identifieri481877 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaEnterocytozoonidaeEnterocytozoon
    ProteomesiUP000001742: Unassembled WGS sequence

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:EBI_22577.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 631631Probable glutamate--tRNA ligase, cytoplasmicPRO_0000388392Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliA9CSZ1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni139 – 1413Glutamate bindingBy similarity
    Regioni311 – 3155Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi144 – 15310"HIGH" regionBy similarity
    Motifi367 – 3715"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    OrthoDBiEOG7MKWFN.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF50715. SSF50715. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9CSZ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLENISKSEF VNFLLEKKHN IEPKTLPILE QHIREQKLDD FFLDFSIDFT    50
    DEELNNFLSK LDYWLKNTKL NSSKADVIFG LLYSCNKFIK LIKNPTFSFV 100
    EIKQFYNDIL NTNKNYIIEY NKKDKGKINI AVEGNVVTRF PPEPSGFLHI 150
    GHIKAALLND LMAKNGKLLI RFDDTNPIKE EKMYENVIIE DLHTLGIKNY 200
    TIVRSSDHFD SLYNYAIQLI QLGLAYCDNT DQLQMREERT KGIPSKNRNT 250
    DIETNLSIFS KMSSGNCLDY CLRAKIDYTN LNKALRDPVI YRHIEKEHNI 300
    TKNKYKIYPT YDFACPIIDS LDGVTLALRT NEYRDRNEQY YWFLEKLNLP 350
    NKPKIYDFSR LNFENTVLSK RQMKFYVDNH FVSGWDDPRL STLRGILRLG 400
    MDIDTLKEYI INQGSSQKSS VISWDKVWSL NKKNIDHKSA RYSAIPKLYC 450
    VECLILDKNN NEIITKTEDI PKFKKNLSLG NKTIIKSSHI LISQEDANIL 500
    NNNEEFTLMN WGNMKVKEKQ IVNGIIIKII LEENLAGDVK TTKNKLTWVN 550
    KENIIEFKIL EYDTLQNDKN TDNLAEKFNT NSKKEEIWLG EKALISVSPK 600
    TYIQIERIGF FICDKPLEFI LIPYTKQKRM R 631
    Length:631
    Mass (Da):73,962
    Last modified:February 5, 2008 - v1
    Checksum:i4DC6852631BEA04D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABGB01000003 Genomic DNA. Translation: EDQ31175.1.
    RefSeqiXP_001828010.1. XM_001827958.1.

    Genome annotation databases

    GeneIDi6004350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ABGB01000003 Genomic DNA. Translation: EDQ31175.1 .
    RefSeqi XP_001828010.1. XM_001827958.1.

    3D structure databases

    ProteinModelPortali A9CSZ1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6004350.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:EBI_22577.

    Phylogenomic databases

    OrthoDBi EOG7MKWFN.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF50715. SSF50715. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi."
      Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J.
      PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H348.
    2. "Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi."
      Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.
      PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H348.

    Entry informationi

    Entry nameiSYEC_ENTBH
    AccessioniPrimary (citable) accession number: A9CSZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3