A9CSU5 (SYQ_ENTBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

ORF Names:

EBI_22570

Organism

Enterocytozoon bieneusi (strain H348) (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

481877 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Enterocytozoonidae › Enterocytozoon › Enterocytozoon bieneusi

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Probable glutamine--tRNA ligase		PRO_0000388382
Regions
	Motif	196 – 206	11	"HIGH" region By similarity
	Motif	436 – 440	5	"KMSKS" region By similarity
Sites
	Binding site	439	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9CSU5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 1F11DE3091ADF9CF	FASTA	691	80,660
	10 20 30 40 50 60 MNLDDKLRGI SLSDEKINEL KTKPNLIKNM EVIVNTGNNF TKMQYSLACI APKNCNLIEL 70 80 90 100 110 120 SNLIEEGYVT NDSLLKGLVK LGKKSKDEYI DFCKKHTYNE QDIINFIRLL KEKKETKATI 130 140 150 160 170 180 SSKLKEQYLY YDTKIFLKEM ENYNLNVPME KYTKNWLDEG EIKFLHKPGE NPQKTKKIME 190 200 210 220 230 240 DHLKRTGGKV ITRFPPEPNG NLHIGHAKAL NLSFEYAKKF NGITYLRFDD TNPKNESNEL 250 260 270 280 290 300 YNGIIEDVKW LGFEPYAITA SSDHFEAMHE MAKTLIKKNK SYVCFCSLEE IRARRSKYQK 310 320 330 340 350 360 ERDEGNDDPL ILSPYRNTSV EQNLIEFEKM LKGEYKDGEA VLRFKMPLKS KNPLMLDLVG 370 380 390 400 410 420 ARIINMVHDR KQKNYIVYPS YEFALCVCDS LEDITHSFCS REFYTRQEPY HWLLQELDMY 430 440 450 460 470 480 EPVQWEFSRL NISNTVLSKR KLTKIVDEGL NWDDPRFYTI KGMRRRGFPA SAINKFVQSV 490 500 510 520 530 540 GITFSETIID VKILESFVLK ELMQIAKKAT CIINPLKVYI NKCTTGIISN EPVDVNNIIY 550 560 570 580 590 600 IDKDDFDETN NSDFLRLTKI QPVGLINLGV LKYIKDESDG IRCELLTVDE CKPNKFIQWL 610 620 630 640 650 660 PNLDNKVELR MYKPLFKSFD PDEIGYMNDI DLTNSLEIID GYVDNTVLNT HIEDKFQFIR 670 680 690 IGFFCCDFDS IINDNQKKLV FNLTLPLNKN Y

References

[1]	"Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi." Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S., Keeling P.J. PLoS ONE 2:E1277-E1277(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.
[2]	"Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi." Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N., Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S. PLoS Pathog. 5:E1000261-E1000261(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABGB01000003 Genomic DNA. Translation: EDQ31158.1.
RefSeq	XP_001827993.1. XM_001827941.1.
3D structure databases
ProteinModelPortal	A9CSU5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6004333.
Organism-specific databases
EuPathDB	MicrosporidiaDB:EBI_22570.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_ENTBH

Accession

Primary (citable) accession number: A9CSU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	February 5, 2008
Last modified:	March 6, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program