ID   A9CMC8_RAT              Unreviewed;      1929 AA.
AC   A9CMC8;
DT   05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT   05-FEB-2008, sequence version 1.
DT   27-MAR-2024, entry version 124.
DE   SubName: Full=Lactase {ECO:0000313|Ensembl:ENSRNOP00000004908.4};
DE   SubName: Full=Lct protein {ECO:0000313|EMBL:BAF94253.1};
GN   Name=Lct {ECO:0000313|EMBL:BAF94253.1,
GN   ECO:0000313|Ensembl:ENSRNOP00000004908.4, ECO:0000313|RGD:620823};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116 {ECO:0000313|EMBL:BAF94253.1};
RN   [1] {ECO:0000313|Ensembl:ENSRNOP00000004908.4, ECO:0000313|Proteomes:UP000002494}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000004908.4,
RC   ECO:0000313|Proteomes:UP000002494};
RX   PubMed=15057822; DOI=10.1038/nature02426;
RG   Rat Genome Sequencing Project Consortium;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2] {ECO:0000313|EMBL:BAF94253.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BUF/Mna {ECO:0000313|EMBL:BAF94253.1};
RX   PubMed=18064521; DOI=10.1007/s00335-007-9078-5;
RA   Akiyama K., Morita H., Suetsugu S., Kuraba S., Numata Y., Yamamoto Y.,
RA   Inui K., Ideura T., Wakisaka N., Nakano K., Oniki H., Takenawa T.,
RA   Matsuyama M., Yoshimura A.;
RT   "Actin-related protein 3 (Arp3) is mutated in proteinuric BUF/Mna rats.";
RL   Mamm. Genome 19:41-50(2008).
RN   [3] {ECO:0000313|Ensembl:ENSRNOP00000004908.4}
RP   IDENTIFICATION.
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000004908.4};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB294578; BAF94253.1; -; Genomic_DNA.
DR   RefSeq; NP_446293.1; NM_053841.1.
DR   Ensembl; ENSRNOT00000004908.7; ENSRNOP00000004908.4; ENSRNOG00000003681.7.
DR   GeneID; 116569; -.
DR   KEGG; rno:116569; -.
DR   CTD; 3938; -.
DR   RGD; 620823; Lct.
DR   GeneTree; ENSGT00940000155324; -.
DR   HOGENOM; CLU_001859_5_3_1; -.
DR   OMA; QITHYKV; -.
DR   OrthoDB; 3373839at2759; -.
DR   TreeFam; TF314803; -.
DR   Proteomes; UP000002494; Chromosome 13.
DR   Bgee; ENSRNOG00000003681; Expressed in jejunum and 4 other cell types or tissues.
DR   GO; GO:0098591; C:external side of apical plasma membrane; IEA:Ensembl.
DR   GO; GO:0008422; F:beta-glucosidase activity; IEA:Ensembl.
DR   GO; GO:0000016; F:lactase activity; IEA:Ensembl.
DR   GO; GO:0140749; F:phlorizin hydrolase activity; IEA:Ensembl.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR   GO; GO:2000892; P:cellobiose catabolic process; IEA:Ensembl.
DR   GO; GO:0005990; P:lactose catabolic process; IEA:Ensembl.
DR   GO; GO:1901733; P:quercetin catabolic process; IEA:Ensembl.
DR   Gene3D; 3.20.20.80; Glycosidases; 4.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR018120; Glyco_hydro_1_AS.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR   PANTHER; PTHR10353:SF36; KLOTHO (MAMMALIAN AGING-ASSOCIATED PROTEIN) HOMOLOG; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 4.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 4.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 3.
PE   4: Predicted;
KW   Glycosidase {ECO:0000256|RuleBase:RU004468};
KW   Hydrolase {ECO:0000256|RuleBase:RU004468};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002494};
KW   Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..1929
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5014084287"
FT   TRANSMEM        1885..1905
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   ACT_SITE        1751
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ   SEQUENCE   1929 AA;  217609 MW;  61735EC3BF194880 CRC64;
     MELPWTALFL STVLLGLSCQ GSDWESDRNF ISAAGPLTND LVLNLNYPPG KQGSDVVSGN
     TDIYICQQPL PSFLSQYFSS LRASQVTHYK VLLSWAQLLP TGSSKNPDQE AVQCYRQLLQ
     SLKDAQLEPM VVLCHQTPPT SSAIQREGAF ADLFADYATL AFQSFGDLVE IWFTFSDLEK
     VIMDLPHKDL KASALQTLSN AHRRAFDIYH RKFSSQGGKL SVVLKAEDIP ELLPDPALAA
     LVQGSVDFLS LDLSYECQSV ATLPQKLSEL QNLEPKVKVF IYTLKLEDCP ATGTSPSSLL
     ISLLEAINKD QIQTVGFDVN AFLSCTSNSE ESPSCSLTDS LALQTEQQQE TAVPSSPGSA
     YQRVWAAFAN QSREERDAFL QDVFPEGFLW GISTGAFNVE GGWAEGGRGP SIWDHYGNLN
     AAEGQATAKV ASDSYHKPAS DVALLRGIRA QVYKFSISWS RLFPTGQKST PNRQGVAYYN
     KLIDRLLDSH IEPMATLFHW DLPQALQEQG GWQNESVVEA FLDYAAFCFS TFGDRVKLWV
     TFHEPWVMSY AGYGTGQHAP AISDPGMASF KVAHLILKAH ARTWHLYDLH HRLQQQGRVG
     IVLNSDWAEP LDRKSPQDLA AAERFLHFML GWFAHPIFVD GDYPTTLRAQ IQHINQQCGH
     PLAQLPEFTE AEKRLLKGSA DFLGLSHYTS RLISKAGRQT CTSSYDNIGG FSQHVDPEWP
     QTASPWIRVV PWGIRRLLRF ASMEYTKGKL PIFLAGNGMP VGEEADLFDD SVRVNYFNLY
     INEVLKAVKE DLVDVRSYIV RSLIDGYEGP LGFSQRFGLY HVNFNDSSRP RTPRKSAYFF
     TSIIEKNGFS AKKVKRNPLP VRADFTSRAR VTDSLPSEVP SKAKIVWEKF SKQPRFERDL
     FYHGTFRDDF LWGVSSSAYQ IEGGWNADGK GPSIWDNFTH TPGNGVKDNA TGDVACDSYH
     QLDADLNILR TLKVKSYRFS ISWSRIFPTG RNSTINKQGV DYYNRLIDSL VDNNIFPMVT
     LFHWDLPQAL QDIGGWENPS LIELFDSYAD FCFKTFGDRV KFWMTFNEPW CHVVLGYSSG
     IFPPSVQEPG WLPYKVSHIV IKAHARVYHT YDEKYRSEQK GVISLSLNTH WAEPKDPGLQ
     RDVEAADRML QFTMGWFAHP IFKNGDYPDV MKWTVGNRSE LQHLASSRLP TFTEEEKNYV
     RGTADVFCIN TYTSVFVQHS TPRLNPPSYD DDMELKLIEM NSSTGVMHPD VPWGTRRLLN
     WIKEEYGNIP IYITENGQGL ENPTLDDTER IFYHKTYINE ALKAYKLDGV DLRGYSAWTL
     MDDFEWLLGY TMRFGLYHVD FNHVSRPRTA RASARYYAEV IANNGMPLAR EDEFLYGEFP
     KGFIWSAASA SYQVEGAWRA DGKGLSIWDT FSHTPLRIGN DDNGDVACDS YHKIAEDVVA
     LQNLGVSHYR FSIAWSRILP DGTTKFINEA GLSYYVRFID ALLAAGITPQ VTIYHWDLPQ
     ALQDVGGWEN ETIVQRFKEY ADVLFQRLGD RVKFWITLNE PFVIAAQGYG TGVSAPGISF
     RPGTAPYIAG HNLIKAHAEA WHLYNDVYRA RQGGTISITI SSDWAEPRDP TNQGDVEAAR
     RYVQFMGGWF AHPIFKNGDY PEVMKTRIRD RSLAAGLNKS RLPEFTESEK SRIKGTFDFF
     GFNHYTTVLA YNLDYPAAFS SFDADRGVAS IADSSWPVSG SFWLKVTPFG FRRILNWLKE
     EYNNPPIYVT ENGVSRRGEP ELNDTDRIYY LRSYINEALK AVQDKVDLRG YTVWSIMDNF
     EWATGFAERF GVHFVNRSDP SLPRIPKASA KFYATIVRCN GFPDPAQGPH PCLQQPEDAA
     PTASPVQSEV PFLGLMLGIA EAQTALYVLF ALLLLGACSL AFLTYKYCRR SKQGNAQPSQ
     HQLSPISSF
//