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Protein

N-acyl homoserine lactonase AiiB

Gene

aiiB

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Kineticsi

  1. KM=15 mM for N-butyryl-(RS)-homoserine lactone (with zinc as cofactor)1 Publication
  2. KM=1.6 mM for N-hexanoyl-(S)-homoserine lactone (with zinc as cofactor)1 Publication
  3. KM=4.6 mM for N-(beta-ketocaproyl)-(RS)-homoserine lactone (with zinc as cofactor)1 Publication
  4. KM=1.0 mM for N-octanoyl-(S)-homoserine lactone (with zinc as cofactor)1 Publication
  5. KM=2.5 mM for N-(3-oxo-octanoyl)-(S)-homoserine lactone (with zinc as cofactor)1 Publication
  6. KM=0.11 mM for N-decanoyl-(S)-homoserine lactone (with zinc as cofactor)1 Publication
  7. KM=7.7 mM for N-t-butyloxycarbonyl-(RS)-homoserine lactone (with zinc as cofactor)1 Publication
  8. KM=0.77 mM for N-carbobenzyloxy-(S)-homoserine lactone (with zinc as cofactor)1 Publication
  9. KM=5.9 mM for N-hexanoyl-(S)-homoserine lactone (with cobalt as cofactor)1 Publication
  10. KM=7.3 mM for N-(beta-ketocaproyl)-(RS)-homoserine lactone (with cobalt as cofactor)1 Publication
  11. KM=7.0 mM for N-(3-oxo-octanoyl)-(S)-homoserine lactone (with cobalt as cofactor)1 Publication
  12. KM=1.6 mM for N-carbobenzyloxy-(S)-homoserine lactone (with cobalt as cofactor)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi111Zinc 1; via tele nitrogen1 Publication1
    Metal bindingi113Zinc 1; via pros nitrogen1 Publication1
    Metal bindingi116Zinc 2; via tele nitrogen1 Publication1
    Metal bindingi191Zinc 1; via tele nitrogen1 Publication1
    Metal bindingi213Zinc 11 Publication1
    Metal bindingi213Zinc 21 Publication1
    Metal bindingi259Zinc 2; via tele nitrogen1 Publication1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciAGRO:ATU6071-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acyl homoserine lactonase AiiB1 Publication (EC:3.1.1.81)
    Short name:
    AHL-lactonase AiiB1 Publication
    Gene namesi
    Name:aiiBImported
    Ordered Locus Names:Atu6071
    ORF Names:AGR_pTi_140
    Encoded oniPlasmid pTiC58CuratedImported
    OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    Proteomesi
    • UP000000813 Componenti: Plasmid Ti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004051251 – 276N-acyl homoserine lactonase AiiBAdd BLAST276

    Structurei

    Secondary structure

    1276
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 16Combined sources13
    Helixi17 – 20Combined sources4
    Beta strandi29 – 31Combined sources3
    Beta strandi38 – 49Combined sources12
    Beta strandi54 – 57Combined sources4
    Helixi74 – 79Combined sources6
    Helixi90 – 96Combined sources7
    Helixi101 – 103Combined sources3
    Beta strandi105 – 108Combined sources4
    Turni114 – 116Combined sources3
    Helixi120 – 122Combined sources3
    Beta strandi124 – 131Combined sources8
    Helixi132 – 143Combined sources12
    Beta strandi148 – 151Combined sources4
    Helixi153 – 159Combined sources7
    Beta strandi166 – 169Combined sources4
    Beta strandi175 – 179Combined sources5
    Beta strandi182 – 201Combined sources20
    Beta strandi203 – 205Combined sources3
    Beta strandi207 – 212Combined sources6
    Helixi218 – 221Combined sources4
    Beta strandi222 – 224Combined sources3
    Helixi234 – 250Combined sources17
    Beta strandi254 – 259Combined sources6
    Helixi261 – 266Combined sources6
    Turni270 – 272Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2R2DX-ray1.75A/B/C/D/E/F1-276[»]
    ProteinModelPortaliA9CKY2.
    SMRiA9CKY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA9CKY2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-beta-lactamase superfamily.Curated

    Phylogenomic databases

    HOGENOMiHOG000094710.
    OMAiPEHEYET.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Metallo-B-lactamas.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9CKY2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGNKLFVLDL GEIRVDENFI IANSTFVTPQ KPTVSSRLID IPVSAYLIQC
    60 70 80 90 100
    TDATVLYDTG CHPECMGTNG RWPAQSQLNA PYIGASECNL PERLRQLGLS
    110 120 130 140 150
    PDDISTVVLS HLHNDHAGCV EYFGKSRLIA HEDEFATAVR YFATGDHSSP
    160 170 180 190 200
    YIVKDIEAWL ATPRNWDLVG RDERERELAP GVNLLNFGTG HASGMLGLAV
    210 220 230 240 250
    RLEKQPGFLL VSDACYTATN YGPPARRAGV LHDTIGYDRT VSHIRQYAES
    260 270
    RSLTVLFGHD REQFASLIKS TDGFYE
    Length:276
    Mass (Da):30,562
    Last modified:January 15, 2008 - v1
    Checksum:i6AD213ADB02C7DA3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE007871 Genomic DNA. Translation: AAK91031.1.
    PIRiAE3236.
    RefSeqiNP_396590.1. NC_003065.3.
    WP_010974862.1. NC_003065.3.

    Genome annotation databases

    EnsemblBacteriaiAAK91031; AAK91031; Atu6071.
    GeneIDi1137394.
    KEGGiatu:Atu6071.
    PATRICi20820368. VBIAgrTum91616_5278.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE007871 Genomic DNA. Translation: AAK91031.1.
    PIRiAE3236.
    RefSeqiNP_396590.1. NC_003065.3.
    WP_010974862.1. NC_003065.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2R2DX-ray1.75A/B/C/D/E/F1-276[»]
    ProteinModelPortaliA9CKY2.
    SMRiA9CKY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAK91031; AAK91031; Atu6071.
    GeneIDi1137394.
    KEGGiatu:Atu6071.
    PATRICi20820368. VBIAgrTum91616_5278.

    Phylogenomic databases

    HOGENOMiHOG000094710.
    OMAiPEHEYET.

    Enzyme and pathway databases

    BioCyciAGRO:ATU6071-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiA9CKY2.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Metallo-B-lactamas.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAHLLB_AGRFC
    AccessioniPrimary (citable) accession number: A9CKY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: January 15, 2008
    Last modified: November 2, 2016
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.