A9CII8 (A9CII8_AGRT5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Name:	icdA EMBL AAK87636.2
Ordered Locus Names:	Atu1870 EMBL AAK87636.2

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP] EMBL AAK87636.2

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRSR PIRSR000108-3 PIRNR PIRNR000108
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRSR PIRSR000108-3 PIRNR PIRNR000108 Manganese PIRSR PIRSR000108-3 PIRNR PIRNR000108 Metal-binding PIRSR PIRSR000108-3 PIRNR PIRNR000108 NADP PIRSR PIRSR000108-4 PIRNR PIRNR000108
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	75 – 77	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	308 – 313	6	NADP By similarity PIRSR PIRSR000108-4
	Region	94 – 100	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	250	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	273	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	77	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	82	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	109	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	132	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	258	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	326	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	139	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	210	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	A9CII8 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: B472E27150AF00FD	FASTA	404	45,068
	10 20 30 40 50 60 MAKIKVANPV VDLDGDEMTR IIWQLIKDKL ILPYLDLDIE YYDLSVENRD ATNDQVTVDA 70 80 90 100 110 120 AHAIKKHGVG IKCATITPDE QRVEEFGLKQ MWKSPNGTIR NILGGVIFRE PIICKNVPRL 130 140 150 160 170 180 VPGWTKPIVV GRHAFGDQYK ATDFKFPGKG KLTIKFVGED GTVIEKDVFD APSAGVALAM 190 200 210 220 230 240 YNLDESIREF ARASMMYGLM RKWPVYLSTK NTILKAYDGR FKDIFEEVYQ TEFKAKFDEV 250 260 270 280 290 300 GITYEHRLID DMVASALKWS GGYVWACKNY DGDVQSDTVA QGFGSLGLMT SVLLSPDGRT 310 320 330 340 350 360 VEAEAAHGTV TRHYRQHQKG QETSTNSIAS IFAWTRGLAH RAKLDDNAEL AKFATTLETV 370 380 390 400 CVDTVESGFM TKDLALLIGP DQPWLSTTAF LDKIDENLKT AMAA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87636.2.
RefSeq	NP_354851.2. NC_003062.2.
3D structure databases
ProteinModelPortal	A9CII8.
SMR	A9CII8. Positions 3-403.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu1870.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1133908.
KEGG	atu:Atu1870.
PATRIC	20813519. VBIAgrTum91616_1884.
Phylogenomic databases
eggNOG	COG0538.
HOGENOM	HOG000019858.
KO	K00031.
OMA	QHQQGKP.
ProtClustDB	PRK08299.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9CII8_AGRT5

Accession

Primary (citable) accession number: A9CII8

Entry history

Integrated into UniProtKB/TrEMBL:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)