A9CHW6 (UREF_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	Atu2397
ORF Names:	AGR_C_4350

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.
Sequence caution	The sequence AAK88134.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Urease accessory protein UreF HAMAP MF_01385		PRO_0000344067

Sequences

	Sequence		Length	Mass (Da)
	A9CHW6 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 1A8E4C257E16C28C	FASTA	223	22,998
	10 20 30 40 50 60 MSAESGVAAL LRLMAWLSPA FPVGGFSYSG GLEKAVEDGR VCDAAGLGGW VETLLRHGSL 70 80 90 100 110 120 WNDAVFLAHA WRSSQDTTAL SETANLGRAL AGSAERYRET VLLGDAFIAA ASAWPHAVLE 130 140 150 160 170 180 LLPKEAPYPV AIGAVAAGHG VPLRETLAAF LHAGVSQIVS AGIRLGVAGQ KDGVAILAAS 190 200 210 220 EAVIEEIAAR AALSTLDDLG SATVIADTAA MRHETQGTRL FRS

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK88134.2. Different initiation.
RefSeq	NP_355349.2. NC_003062.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9CHW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1134435.
GenomeReviews	Gene locus Atu2397 in contig AE007869_GR.
KEGG	atu:Atu2397.
PATRIC	20814575. VBIAgrTum91616_2406.
Phylogenomic databases
eggNOG	COG0830.
HOGENOM	HBG709295.
OMA	WLSPAFP.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF_AGRT5

Accession

Primary (citable) accession number: A9CHW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program